USP8 sirna

USP8 siRNA (Mouse)

Cat. Num. AAA8235067

• Gene Names: Usp8; Ubpy; AI574262; AW557536; mKIAA0055
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USP8; USP8 siRNA (Mouse); KIAA0055; UBPY; Ubiquitin carboxyl-terminal hydrolase 8; Deubiquitinating enzyme 8; Ubiquitin isopeptidase Y; mUBPy; Ubiquitin thioesterase 8; Ubiquitin-specific-processing protease 8; USP8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: USP8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1080

• Applicable Applications for USP8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USP8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USP8 sirna

siRNA to inhibit USP8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31981044
• NCBI GeneID: 84092
• NCBI Accession #: NP_062703.2
• NCBI GenBank Nucleotide #: NM_019729.3
• UniProt Accession #: Q80U87; Q80YP2; Q8R0D3; Q9EQU1; Q9WVP5; A2AI53
• Molecular Weight: 122,611 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 8 isoform 2
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 8
• NCBI Official Symbol: Usp8
• NCBI Official Synonym Symbols: Ubpy; AI574262; AW557536; mKIAA0055
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 8
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 8
• UniProt Gene Name: Usp8
• UniProt Synonym Gene Names: Kiaa0055; Ubpy; mUBPy
• UniProt Entry Name: UBP8_MOUSE

Uniprot Description

USP8: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1. Upon growth stimulation in starved human fibroblasts. Decreases in response to growth arrest induced by cell-cell contact. Belongs to the peptidase C19 family.

Protein type: Protease; EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease

Cellular Component: nucleoplasm; extrinsic to plasma membrane; Golgi apparatus; membrane; cytoplasm; early endosome; acrosome; plasma membrane; midbody; intracellular; nucleus; cytosol; endosome

Molecular Function: peptidase activity; protein binding; hydrolase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SH3 domain binding; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; cytokinesis after mitosis; endosome organization and biogenesis; Ras protein signal transduction; proteolysis; cell cycle