EMG1 sirna

EMG1 siRNA (Mouse)

Cat. Num. AAA8234862

• Gene Names: Emg1; C2f; Grcc2f
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EMG1; EMG1 siRNA (Mouse); C2F; GRCC2F; Ribosomal RNA small subunit methyltransferase NEP1; 18S rRNA; pseudouridine-N1-)-methyltransferase NEP1; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1; EMG1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EMG1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 244

• Applicable Applications for EMG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EMG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EMG1 sirna

siRNA to inhibit EMG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7305109
• NCBI GeneID: 14791
• NCBI Accession #: NP_038564.1
• NCBI GenBank Nucleotide #: NM_013536.2
• UniProt Accession #: O35130
• Molecular Weight: 26,974 Da
• NCBI Official Full Name: ribosomal RNA small subunit methyltransferase NEP1
• NCBI Official Synonym Full Names: EMG1 nucleolar protein homolog (S. cerevisiae)
• NCBI Official Symbol: Emg1
• NCBI Official Synonym Symbols: C2f; Grcc2f
• NCBI Protein Information: ribosomal RNA small subunit methyltransferase NEP1
• UniProt Protein Name: Ribosomal RNA small subunit methyltransferase NEP1
• UniProt Gene Name: Emg1
• UniProt Entry Name: NEP1_MOUSE

Uniprot Description

C2F: Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of pseudouridine at position 1248 (Psi1248) in 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) in position 1248 in 18S rRNA. Is not able to methylate uridine at this position. Defects in EMG1 are the cause of Bowen-Conradi syndrome (BWCNS). BWCNS is a combination of malformations characterized in newborns by low birth weight, microcephaly, mild joint restriction, a prominent nose, micrognathia, fifth finger clinodactyly, and 'rocker-bottom' feet. The syndrome is transmitted as an autosomal recessive trait. The prognosis is poor, with all infants dying within the first few months of life. Belongs to the NEP1 family.

Protein type: Nucleolus; RNA-binding; EC 2.1.1.-

Cellular Component: small subunit processome; nucleolus; nucleus

Molecular Function: methyltransferase activity; monomethylamine methyltransferase activity; trimethylamine methyltransferase activity; RNA binding; tRNA (cytosine)-methyltransferase activity; RNA methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; tRNA (guanine) methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; selenocysteine methyltransferase activity; arginine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; protein-arginine N-methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; hydroxyneurosporene-O-methyltransferase activity; transferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; C-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; tRNA (adenine)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; protein-leucine O-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; rRNA binding; mRNA methyltransferase activity; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity

Biological Process: methylation; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); blastocyst development; nucleologenesis; rRNA methylation; ribosome biogenesis and assembly; rRNA processing