CHRNB2 sirna

CHRNB2 siRNA (Human)

Cat. Num. AAA8234543

• Gene Names: CHRNB2; EFNL3; nAChRB2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHRNB2; CHRNB2 siRNA (Human); Neuronal acetylcholine receptor subunit beta-2; CHRNB2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CHRNB2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 502

• Applicable Applications for CHRNB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHRNB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHRNB2 sirna

siRNA to inhibit CHRNB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502833
• NCBI GeneID: 1141
• NCBI Accession #: NP_000739.1
• NCBI GenBank Nucleotide #: NM_000748.2
• UniProt Accession #: P17787; Q9UEH9
• Molecular Weight: 57,019 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit beta-2
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, beta 2 (neuronal)
• NCBI Official Symbol: CHRNB2
• NCBI Official Synonym Symbols: EFNL3; nAChRB2
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-2
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-2
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNB2
• UniProt Entry Name: ACHB2_HUMAN

NCBI Description

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]

Uniprot Description

nAChRB2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3). ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 2/CHRNB2 sub-subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, cation; Receptor, misc.; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; integral to membrane; plasma membrane; cell junction; external side of plasma membrane

Molecular Function: protein heterodimerization activity; acetylcholine receptor activity; drug binding; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding; ligand-gated ion channel activity

Biological Process: response to nicotine; positive regulation of dopamine secretion; protein heterooligomerization; regulation of dendrite morphogenesis; behavioral response to nicotine; locomotory behavior; regulation of circadian sleep/wake cycle, non-REM sleep; signal transduction; sensory perception of pain; regulation of dopamine secretion; synaptic transmission, cholinergic; synaptic transmission; smooth muscle contraction; regulation of synaptic transmission, dopaminergic; sensory perception of sound; visual perception; conditioned taste aversion; calcium ion transport; positive regulation of B cell proliferation; visual learning; synaptic transmission involved in micturition; associative learning; regulation of circadian sleep/wake cycle, REM sleep; vestibulocochlear nerve development; regulation of action potential; B cell activation; regulation of dopamine metabolic process; positive regulation of synaptic transmission, dopaminergic; central nervous system projection neuron axonogenesis; social behavior; negative regulation of action potential; learning; optic nerve morphogenesis; response to cocaine; memory; neurological system process; membrane depolarization; response to ethanol; regulation of synaptogenesis; response to hypoxia; ion transport; cognition; lateral geniculate nucleus development

Disease: Epilepsy, Nocturnal Frontal Lobe, 3