FAM134B sirna

FAM134B siRNA (Mouse)

Cat. Num. AAA8234239

• Gene Names: Fam134b; AU015349; 1810015C04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FAM134B; FAM134B siRNA (Mouse); Protein FAM134B; FAM134B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FAM134B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 356

• Applicable Applications for FAM134B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FAM134B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FAM134B sirna

siRNA to inhibit FAM134B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 474451725
• NCBI GeneID: 66270
• NCBI Accession #: NP_001264244.1
• NCBI GenBank Nucleotide #: NM_001277315.1
• UniProt Accession #: Q8VE91; Q7TMY5; Q9CUJ4; Q9D8Z5
• Molecular Weight: 38,153 Da
• NCBI Official Full Name: protein FAM134B isoform 2
• NCBI Official Synonym Full Names: family with sequence similarity 134, member B
• NCBI Official Symbol: Fam134b
• NCBI Official Synonym Symbols: AU015349; 1810015C04Rik
• NCBI Protein Information: protein FAM134B
• UniProt Protein Name: Protein FAM134B
• Protein Family: Reticulophagy receptor
• UniProt Gene Name: Fam134b
• UniProt Entry Name: F134B_MOUSE

Uniprot Description

FAM134B: Required for long-term survival of nociceptive and autonomic ganglion neurons. Defects in FAM134B are the cause of hereditary sensory and autonomic neuropathy type 2B (HSAN2B). A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN2B is an autosomal recessive disorder characterized by impairment of pain, temperature and touch sensation. Onset occurs in the first or second decade, with impaired nociception and progressive mutilating ulceration of the hands and feet with osteomyelitis and acroosteolysis. Amputations of the hands and feet are common. Autonomic dysfunction includes hyperhidrosis, urinary incontinence, and slow pupillary light response. Belongs to the FAM134 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: Golgi apparatus; cis-Golgi network; membrane; integral to membrane

Biological Process: sensory perception of pain