LRP1 sirna

LRP1 siRNA (Human)

Cat. Num. AAA8234162

• Gene Names: LRP1; APR; LRP; A2MR; CD91; APOER; LRP1A; TGFBR5; IGFBP3R
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LRP1; LRP1 siRNA (Human); A2MR; APR; Prolow-density lipoprotein receptor-related protein 1; LRP-1; Alpha-2-macroglobulin receptor; Apolipoprotein E receptor; APOER; CD antigen CD91; LRP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LRP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 4544

• Applicable Applications for LRP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LRP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LRP1 sirna

siRNA to inhibit LRP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 126012562
• NCBI GeneID: 4035
• NCBI Accession #: NP_002323.2
• NCBI GenBank Nucleotide #: NM_002332.2
• UniProt Accession #: Q07954; Q2PP12; Q86SW0; Q8IVG8
• Molecular Weight: 31,631 Da
• NCBI Official Full Name: prolow-density lipoprotein receptor-related protein 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein 1
• NCBI Official Symbol: LRP1
• NCBI Official Synonym Symbols: APR; LRP; A2MR; CD91; APOER; LRP1A; TGFBR5; IGFBP3R
• NCBI Protein Information: prolow-density lipoprotein receptor-related protein 1
• UniProt Protein Name: Prolow-density lipoprotein receptor-related protein 1
• Protein Family: Low-density lipoprotein receptor-related protein
• UniProt Gene Name: LRP1
• UniProt Synonym Gene Names: A2MR; APR; LRP-1; A2MR; APOER; LRP-85; LRP-515; LRPICD
• UniProt Entry Name: LRP1_HUMAN

NCBI Description

The protein encoded by this gene is an endocytic receptor involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients. [provided by RefSeq, Jan 2010]

Uniprot Description

LRP1: a receptor involved in endocytosis, phagocytosis of apoptotic cells, and neural signaling. Modulates cellular events including APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling and neurotransmission. Involved in the plasma clearance of chylomicron remnants and activated alpha 2-macroglobulin, as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Plays a fundamental role in cellular signaling pathways in the brain. Mediates neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. A type I membrane protein. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus. Most abundant in liver, brain and lung.

Protein type: Cell surface; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: focal adhesion; cell soma; clathrin-coated vesicle; integral to plasma membrane; lysosomal membrane; dendrite; cytoplasm; nucleolus; plasma membrane; coated pit; receptor complex; endosome

Molecular Function: protein binding; lipoprotein transporter activity; protease binding; protein complex binding; apolipoprotein binding; receptor activity; calcium ion binding

Biological Process: cholesterol metabolic process; phototransduction, visible light; receptor-mediated endocytosis; regulation of phospholipase A2 activity; negative regulation of Wnt receptor signaling pathway; lipoprotein metabolic process; negative regulation of smooth muscle cell migration; positive regulation of protein transport; cell proliferation; positive regulation of lipid transport; lipoprotein transport; regulation of actin cytoskeleton organization and biogenesis; protein kinase C activation; negative regulation of neuron apoptosis; retinoid metabolic process; regulation of cholesterol transport; apoptotic cell clearance; aging