GUCA1A sirna

GUCA1A siRNA (Human)

Cat. Num. AAA8234156

• Gene Names: GUCA1A; COD3; GCAP; GUCA; GCAP1; GUCA1; CORD14; C6orf131; dJ139D8.6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GUCA1A; GUCA1A siRNA (Human); C6orf131; GCAP; GCAP1; GUCA1; Guanylyl cyclase-activating protein 1; GCAP 1; Guanylate cyclase activator 1A; GUCA1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GUCA1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 201

• Applicable Applications for GUCA1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GUCA1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GUCA1A sirna

siRNA to inhibit GUCA1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40254415
• NCBI GeneID: 2978
• NCBI Accession #: NP_000400.2
• NCBI GenBank Nucleotide #: NM_000409.3
• UniProt Accession #: P43080; Q7Z6T1; Q9NU14; B3KWT4
• Molecular Weight: 22,920 Da
• NCBI Official Full Name: guanylyl cyclase-activating protein 1
• NCBI Official Synonym Full Names: guanylate cyclase activator 1A (retina)
• NCBI Official Symbol: GUCA1A
• NCBI Official Synonym Symbols: COD3; GCAP; GUCA; GCAP1; GUCA1; CORD14; C6orf131; dJ139D8.6
• NCBI Protein Information: guanylyl cyclase-activating protein 1
• UniProt Protein Name: Guanylyl cyclase-activating protein 1
• Protein Family: Guanylyl cyclase-activating protein
• UniProt Gene Name: GUCA1A
• UniProt Synonym Gene Names: C6orf131; GCAP; GCAP1; GUCA1; GCAP 1
• UniProt Entry Name: GUC1A_HUMAN

NCBI Description

This gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase activating protein 1 (GCAP1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in this gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. Mutations in this gene prohibit the inactivation of RetGC1 at high Ca(2+) concentrations; causing the constitutive activation of RetGC1 and, presumably, increased cell death. This gene is expressed in retina and spermatagonia. [provided by RefSeq, Feb 2009]

Uniprot Description

GUCA1A: Stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure. Defects in GUCA1A are the cause of cone dystrophy type 3 (COD3). COD3 is an autosomal dominant cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies, in which some loss of peripheral vision also occurs.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: photoreceptor inner segment; plasma membrane

Molecular Function: calcium sensitive guanylate cyclase activator activity; calcium ion binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; positive regulation of guanylate cyclase activity; signal transduction

Disease: Cone Dystrophy 3