MADD sirna

MADD siRNA (Mouse)

Cat. Num. AAA8233739

• Gene Names: Madd; IG20; 9630059K23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MADD; MADD siRNA (Mouse); KIAA0358; MAP kinase-activating death domain protein; Rab3 GDP/GTP exchange factor; MADD sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MADD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1577

• Applicable Applications for MADD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MADD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MADD sirna

siRNA to inhibit MADD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 295148169
• NCBI GeneID: 228355
• NCBI Accession #: NP_001171190.1
• NCBI GenBank Nucleotide #: NM_001177719.1
• UniProt Accession #: Q80U28; Q3TQK3; Q6P4I6; Q80W52; Q80WL3; Q80WL4; Q80WL5; Q80WL6; Q80WL7; Q80WL8; Q80WL9; Q80WM0
• Molecular Weight: 174,892 Da
• NCBI Official Full Name: MAP kinase-activating death domain protein isoform 10
• NCBI Official Synonym Full Names: MAP-kinase activating death domain
• NCBI Official Symbol: Madd
• NCBI Official Synonym Symbols: IG20; 9630059K23Rik
• NCBI Protein Information: MAP kinase-activating death domain protein
• UniProt Protein Name: MAP kinase-activating death domain protein
• Protein Family: Malonyl-S-ACP:biotin-protein carboxyltransferase
• UniProt Gene Name: Madd
• UniProt Entry Name: MADD_MOUSE

Uniprot Description

MADD: a guanine nucleotide exchange factor (GEF) that activates RAB3A/C/D. A GEF for Rab27A/B in melanocytes and during isoproterenol (IPR)-stimulated amylase release from rat parotid acinar cells. Plays a significant role in regulating cell proliferation, survival and death through alternatively spliced isoforms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis). Interacts with the death domain of TNFRSF1A through its own death domain. Highly expressed in fetal brain and kidney, adult testis, ovary, brain and heart. Overexpression of MADD activates the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK). Expression of the MADD death domain stimulates both the ERK and c-JUN N-terminal kinase MAP kinases and induces the phosphorylation of cytosolic phospholipase A2. Isoform 1 is pro-apoptotic, isoform 5 is anti-apoptotic and isoform 3 and isoform 4 have no effect. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines. Isoform 5 is associated with increased cell proliferation and isoform 2 with decreased proliferation. Belongs to the MADD family. Seven isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rab; Adaptor/scaffold; Membrane protein, integral; GEFs

Cellular Component: synaptic vesicle; membrane; cytoplasm; plasma membrane; integral to membrane; synapse; nucleus; cytosol

Molecular Function: guanyl-nucleotide exchange factor activity; Rab guanyl-nucleotide exchange factor activity

Biological Process: regulation of Rab protein signal transduction; negative regulation of growth hormone secretion; regulation of apoptosis; activation of MAPK activity; apoptosis; regulation of cell cycle; positive regulation of GTPase activity