GGA2 sirna

GGA2 siRNA (Human)

Cat. Num. AAA8232998

• Gene Names: GGA2; VEAR
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GGA2; GGA2 siRNA (Human); KIAA1080; ADP-ribosylation factor-binding protein GGA2; Gamma-adaptin-related protein 2; Golgi-localized. gamma ear-containing. ARF-binding protein 2; VHS domain and ear domain of gamma-adaptin; Vear; GGA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GGA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 613

• Applicable Applications for GGA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GGA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GGA2 sirna

siRNA to inhibit GGA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9558751
• NCBI GeneID: 23062
• NCBI Accession #: NP_055859.1
• NCBI GenBank Nucleotide #: NM_015044.4
• UniProt Accession #: Q9UJY4; O14564; Q9NYN2; Q9UPS2; D3DWF0
• Molecular Weight: 67,150 Da
• NCBI Official Full Name: ADP-ribosylation factor-binding protein GGA2
• NCBI Official Synonym Full Names: golgi-associated, gamma adaptin ear containing, ARF binding protein 2
• NCBI Official Symbol: GGA2
• NCBI Official Synonym Symbols: VEAR
• NCBI Protein Information: ADP-ribosylation factor-binding protein GGA2
• UniProt Protein Name: ADP-ribosylation factor-binding protein GGA2
• Protein Family: ADP-ribosylation factor-binding protein
• UniProt Gene Name: GGA2
• UniProt Synonym Gene Names: KIAA1080; Vear
• UniProt Entry Name: GGA2_HUMAN

NCBI Description

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq, Jul 2008]

Uniprot Description

GGA2: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Belongs to the GGA protein family.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: Golgi apparatus; clathrin adaptor complex; clathrin-coated vesicle; endosome membrane; trans-Golgi network

Molecular Function: ADP-ribosylation factor binding; protein binding

Biological Process: vesicle-mediated transport; intracellular protein transport