HR sirna

HR siRNA (Human)

Cat. Num. AAA8232392

• Gene Names: HR; AU; MUHH; ALUNC; HYPT4; MUHH1; HSA277165
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HR; HR siRNA (Human); Protein hairless; HR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1189

• Applicable Applications for HR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HR sirna

siRNA to inhibit HR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22547204
• NCBI GeneID: 55806
• NCBI Accession #: NP_005135.2
• NCBI GenBank Nucleotide #: NM_005144.4
• UniProt Accession #: O43593; Q6GS30; Q96H33; Q9NPE1
• Molecular Weight: 121,857 Da
• NCBI Official Full Name: lysine-specific demethylase hairless isoform a
• NCBI Official Synonym Full Names: hair growth associated
• NCBI Official Symbol: HR
• NCBI Official Synonym Symbols: AU; MUHH; ALUNC; HYPT4; MUHH1; HSA277165
• NCBI Protein Information: lysine-specific demethylase hairless
• UniProt Protein Name: Lysine-specific demethylase hairless
• Protein Family: Hrp65 protein
• UniProt Gene Name: HR
• UniProt Entry Name: HAIR_HUMAN

NCBI Description

This gene encodes a protein that is involved in hair growth. This protein functions as a transcriptional corepressor of multiple nuclear receptors, including thyroid hormone receptor, the retinoic acid receptor-related orphan receptors and the vitamin D receptors, and it interacts with histone deacetylases. The translation of this protein is modulated by a regulatory open reading frame (ORF) that exists upstream of the primary ORF. Mutations in this upstream ORF cause Marie Unna hereditary hypotrichosis (MUHH), an autosomal dominant form of genetic hair loss. Mutations in this gene also cause autosomal recessive congenital alopecia and atrichia with papular lesions, other diseases resulting in hair loss. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

HR: May act as a transcription factor that could act on to regulate one of the phases of hair growth. Defects in HR are the cause of alopecia universalis congenita (ALUNC). ALUNC is a rare autosomal recessive form of hair loss characterized by hair follicles without hair. Defects in HR are the cause of atrichia with papular lesions (APL); also known as congenital atrichia. APL is an autosomal recessive disease characterized by papillary lesions over most of the body and almost complete absence of hair. Defects in HR are the cause of hypotrichosis type 4 (HYPT4). An autosomal dominant condition characterized by reduced amount of hair, alopecia, little or no eyebrows, eyelashes or body hair, and coarse, wiry, twisted hair in early childhood. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nuclear body; nucleus

Molecular Function: DNA binding; metal ion binding; oxidoreductase activity; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of transcription, DNA-dependent

Disease: Hypotrichosis 4; Alopecia Universalis Congenita; Atrichia With Papular Lesions