PARD3 sirna

PARD3 siRNA (Mouse)

Cat. Num. AAA8232238

• Gene Names: Pard3; Asip; Par3; Phip; Par-3; Pard-3; Pard3a; AA960621; AI256638; D8Ertd580e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PARD3; PARD3 siRNA (Mouse); PAR3; Partitioning defective 3 homolog; PAR-3; PARD-3; Atypical PKC isotype-specific-interacting protein; ASIP; Ephrin-interacting protein; PHIP; PARD3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PARD3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 606

• Applicable Applications for PARD3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PARD3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PARD3 sirna

siRNA to inhibit PARD3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61888842
• NCBI GeneID: 93742
• NCBI Accession #: NP_001013598.1
• NCBI GenBank Nucleotide #: NM_001013580.3
• UniProt Accession #: Q99NH2; Q58T10; Q58T11; Q8CB21
• Molecular Weight: 66,609 Da
• NCBI Official Full Name: partitioning defective 3 homolog isoform 2
• NCBI Official Synonym Full Names: par-3 family cell polarity regulator
• NCBI Official Symbol: Pard3
• NCBI Official Synonym Symbols: Asip; Par3; Phip; Par-3; Pard-3; Pard3a; AA960621; AI256638; D8Ertd580e
• NCBI Protein Information: partitioning defective 3 homolog
• UniProt Protein Name: Partitioning defective 3 homolog
• Protein Family: Partitioning defective
• UniProt Gene Name: Pard3
• UniProt Synonym Gene Names: Par3; PAR-3; PARD-3; ASIP; PHIP
• UniProt Entry Name: PARD3_MOUSE

Uniprot Description

PAR3-alpha: an adapter protein involved in asymmetrical cell division, and cell polarization processes. Polarity complex proteins, including PARD3, are essential for PIP3-induced receptor tyrosine kinase recycling. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. PDZ 1 domain modulates interactions with JAM1, PARD6A and PARD6B; PDZ 2 domain mediates interaction with membranes containing phosphoinositol lipids; PDZ 3 domain regulates interactions with PTEN (via C-terminus). Belongs to the PAR3 family. 10 isoforms of the human protein are produced by alternative splicing. Isoform 2, but not at least isoform 3 interacts with PRKCZ.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: protein complex; tight junction; lateral loop; intercellular junction; cell cortex; internode region of axon; cell-cell adherens junction; adherens junction; cytoskeleton; cell soma; membrane; apical part of cell; cytoplasm; plasma membrane; spindle; intracellular; cell junction

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; protein phosphatase binding; lipid binding; phosphatidylinositol 3-phosphate binding

Biological Process: positive regulation of myelination; regulation of actin filament-based process; cell-cell adhesion; centrosome localization; cell division; establishment and/or maintenance of cell polarity; microtubule cytoskeleton organization and biogenesis; protein targeting to membrane; cell cycle; cell differentiation; myelination in the peripheral nervous system