PKD2 sirna

PKD2 siRNA (Mouse)

Cat. Num. AAA8231943

• Gene Names: Pkd2; PC2; TRPP2; C030034P18Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PKD2; PKD2 siRNA (Mouse); Polycystin-2; Polycystic kidney disease 2 protein homolog; PKD2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PKD2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 966

• Applicable Applications for PKD2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PKD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PKD2 sirna

siRNA to inhibit PKD2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 164519057
• NCBI GeneID: 18764
• NCBI Accession #: NP_032887.3
• NCBI GenBank Nucleotide #: NM_008861.3
• UniProt Accession #: O35245; Q8BPR6; Q9ES37; Q9QWP0; Q9Z193; Q9Z194; C0KJK2; E9Q4F6; E9QQA3
• Molecular Weight: 98,082 Da
• NCBI Official Full Name: polycystin-2
• NCBI Official Synonym Full Names: polycystic kidney disease 2
• NCBI Official Symbol: Pkd2
• NCBI Official Synonym Symbols: PC2; TRPP2; C030034P18Rik
• NCBI Protein Information: polycystin-2
• UniProt Protein Name: Polycystin-2
• Protein Family: Polycystin
• UniProt Gene Name: Pkd2
• UniProt Entry Name: PKD2_MOUSE

Uniprot Description

PKD2: Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Functions as a calcium permeable cation channel. Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2). ADPKD2 is a disorder characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. It represents approximately 15% of the cases of autosomal dominant polycystic kidney disease. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy. Belongs to the polycystin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; nonmotile primary cilium; endoplasmic reticulum; integral to membrane; intercellular junction; cytosol; cilium; filamentous actin; cell projection; membrane; lamellipodium; cytoplasm; basal plasma membrane; plasma membrane; motile primary cilium; basal cortex

Molecular Function: voltage-gated cation channel activity; identical protein binding; actinin binding; protein homodimerization activity; channel activity; calcium channel activity; metal ion binding; phosphoprotein binding; voltage-gated ion channel activity; calcium ion binding; alpha-actinin binding; muscle alpha-actinin binding; ATPase binding; calcium-induced calcium release activity; voltage-gated calcium channel activity; protein binding; potassium channel activity; cytoskeletal protein binding; voltage-gated sodium channel activity; HLH domain binding; receptor binding

Biological Process: positive regulation of inositol-1,4,5-triphosphate receptor activity; embryonic placenta development; cytoplasmic sequestering of transcription factor; positive regulation of nitric oxide biosynthetic process; regulation of cAMP metabolic process; heart development; detection of mechanical stimulus; liver development; JAK-STAT cascade; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; regulation of cell proliferation; cellular calcium ion homeostasis; elevation of cytosolic calcium ion concentration; transport; calcium ion transport; release of sequestered calcium ion into cytosol; ion transport; positive regulation of transcription from RNA polymerase II promoter; heart looping; kidney development; determination of left/right symmetry; cell cycle arrest; transmembrane transport