PDGFB sirna

PDGFB siRNA (Mouse)

Cat. Num. AAA8231210

• Gene Names: Pdgfb; Sis; PDGF-B
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDGFB; PDGFB siRNA (Mouse); SIS; Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; PDGFB sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PDGFB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 241

• Applicable Applications for PDGFB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PDGFB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDGFB sirna

siRNA to inhibit PDGFB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40538817
• NCBI GeneID: 18591
• NCBI Accession #: NP_035187.2
• NCBI GenBank Nucleotide #: NM_011057.3
• UniProt Accession #: P31240; Q6P3C4
• Molecular Weight: 27,382 Da
• NCBI Official Full Name: platelet-derived growth factor subunit B
• NCBI Official Synonym Full Names: platelet derived growth factor, B polypeptide
• NCBI Official Symbol: Pdgfb
• NCBI Official Synonym Symbols: Sis; PDGF-B
• NCBI Protein Information: platelet-derived growth factor subunit B
• UniProt Protein Name: Platelet-derived growth factor subunit B
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: Pdgfb
• UniProt Synonym Gene Names: Sis; PDGF subunit B
• UniProt Entry Name: PDGFB_MOUSE

Uniprot Description

PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell surface; membrane; basolateral plasma membrane; cytoplasm; extracellular region

Molecular Function: collagen binding; identical protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; superoxide-generating NADPH oxidase activator activity; receptor binding; chemoattractant activity

Biological Process: embryonic placenta development; peptidyl-tyrosine phosphorylation; positive regulation of cyclin-dependent protein kinase activity; positive regulation of smooth muscle cell proliferation; heart development; multicellular organismal development; positive regulation of transcription, DNA-dependent; positive regulation of fibroblast growth factor receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of fibroblast proliferation; positive chemotaxis; positive regulation of MAPKKK cascade; negative regulation of vasodilation; positive regulation of glomerular filtration; positive regulation of cell proliferation; response to wounding; positive regulation of mitotic cell cycle, embryonic; regulation of peptidyl-tyrosine phosphorylation; negative regulation of cell migration; substrate-bound cell migration; positive regulation of mitosis; positive regulation of chemotaxis; activation of protein kinase B; positive regulation of blood vessel endothelial cell migration; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of cell division; activation of protein kinase activity; positive regulation of vasoconstriction; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; negative regulation of transcription, DNA-dependent; blood coagulation; actin cytoskeleton organization and biogenesis; positive regulation of DNA replication; positive regulation of cell migration