RNF10 sirna

RNF10 siRNA (Mouse)

Cat. Num. AAA8230649

• Gene Names: Rnf10; RIE2; AA675014; mKIAA0262
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF10; RNF10 siRNA (Mouse); KIAA0262; RIE2; SID2705; RING finger protein 10; Sid 2705; RNF10 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RNF10 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 805

• Applicable Applications for RNF10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RNF10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF10 sirna

siRNA to inhibit RNF10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 698980063
• NCBI GeneID: 50849
• NCBI Accession #: NP_001289377.1
• NCBI GenBank Nucleotide #: NM_001302448.1
• UniProt Accession #: Q3UIW5; Q6PDS8; Q6ZQE8; Q91YZ9; Q9R0P0
• Molecular Weight: 77,854 Da
• NCBI Official Full Name: RING finger protein 10 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 10
• NCBI Official Symbol: Rnf10
• NCBI Official Synonym Symbols: RIE2; AA675014; mKIAA0262
• NCBI Protein Information: RING finger protein 10
• UniProt Protein Name: RING finger protein 10
• Protein Family: RING finger protein
• UniProt Gene Name: Rnf10
• UniProt Synonym Gene Names: Kiaa0262; Rie2; Sid2705
• UniProt Entry Name: RNF10_MOUSE

NCBI Description

The protein encoding this gene is a member of the really interesting new gene finger protein family. Members of this family contain protein motifs similar to zinc finger domains and are involved in many processes that include transcriptional regulation, DNA repair and signal transduction. Expression of this gene is upregulated during neuronal differentiation of cultured cells, and inhibition of its expression impairs differentiation and cell cycle exit, providing evidence for a function in neuronal differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

RNF10: Transcriptional factor involved in the regulation of MAG expression. Participates in the peripheral nerve development and Schawnn cell differentiation. Belongs to the RNF10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; zinc ion binding; metal ion binding; ubiquitin-protein ligase activity

Biological Process: positive regulation of myelination; protein autoubiquitination; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter