ADAM11 sirna

ADAM11 siRNA (Human)

Cat. Num. AAA8230400

• Gene Names: ADAM11; MDC
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADAM11; ADAM11 siRNA (Human); MDC; Disintegrin and metalloproteinase domain-containing protein 11; ADAM 11; Metalloproteinase-like. disintegrin-like. and cysteine-rich protein; ADAM11 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADAM11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 769

• Applicable Applications for ADAM11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADAM11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADAM11 sirna

siRNA to inhibit ADAM11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4585710
• NCBI GeneID: 4185
• NCBI Accession #: NP_002381.2
• NCBI GenBank Nucleotide #: NM_002390.4
• UniProt Accession #: O75078; Q14808; Q14809; Q14810; AAB29191
• Molecular Weight: 57,911 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 11 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 11
• NCBI Official Symbol: ADAM11
• NCBI Official Synonym Symbols: MDC
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 11
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 11
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM11
• UniProt Synonym Gene Names: MDC; ADAM 11; MDC
• UniProt Entry Name: ADA11_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene represents a candidate tumor suppressor gene for human breast cancer based on its location within a minimal region of chromosome 17q21 previously defined by tumor deletion mapping. [provided by RefSeq, Dec 2014]

Uniprot Description

ADAM11: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: extracellular matrix; integral to membrane; plasma membrane

Molecular Function: integrin binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity

Biological Process: integrin-mediated signaling pathway; proteolysis