CCNT1 sirna

CCNT1 siRNA (Human)

Cat. Num. AAA8230019

• Gene Names: CCNT1; CCNT; CYCT1; HIVE1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCNT1; CCNT1 siRNA (Human); Cyclin-T1; CycT1; Cyclin-T; CCNT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCNT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 726

• Applicable Applications for CCNT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCNT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCNT1 sirna

siRNA to inhibit CCNT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17978466
• NCBI GeneID: 904
• NCBI Accession #: NP_001231.2
• NCBI GenBank Nucleotide #: NM_001240.3
• UniProt Accession #: O60563; O60581; A9XU13; E7EX76
• Molecular Weight: 21,206 Da
• NCBI Official Full Name: cyclin-T1 isoform a
• NCBI Official Synonym Full Names: cyclin T1
• NCBI Official Symbol: CCNT1
• NCBI Official Synonym Symbols: CCNT; CYCT1; HIVE1
• NCBI Protein Information: cyclin-T1
• UniProt Protein Name: Cyclin-T1
• Protein Family: Cyclin
• UniProt Gene Name: CCNT1
• UniProt Synonym Gene Names: CycT1; Cyclin-T
• UniProt Entry Name: CCNT1_HUMAN

NCBI Description

This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

CCNT1: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with MDFIC. Ubiquitously expressed. Belongs to the cyclin family. Cyclin C subfamily.

Protein type: Transcription initiation complex; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: nucleoplasm; transcription elongation factor complex b; nucleolus; nucleus

Molecular Function: protein binding; snRNA binding; DNA binding; chromatin binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; cell cycle; protein amino acid phosphorylation; cell division; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of cyclin-dependent protein kinase activity