RBP3 sirna

RBP3 siRNA (Human)

Cat. Num. AAA8229796

• Gene Names: RBP3; IRBP; RBPI; RP66; D10S64; D10S65; D10S66
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RBP3; RBP3 siRNA (Human); Retinol-binding protein 3; Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein; RBP3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RBP3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1247

• Applicable Applications for RBP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RBP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RBP3 sirna

siRNA to inhibit RBP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4506453
• NCBI GeneID: 5949
• NCBI Accession #: NP_002891.1
• NCBI GenBank Nucleotide #: NM_002900.2
• UniProt Accession #: P10745; Q0QD34; Q5VSR0; Q8IXN0
• Molecular Weight: 135,363 Da
• NCBI Official Full Name: retinol-binding protein 3
• NCBI Official Synonym Full Names: retinol binding protein 3, interstitial
• NCBI Official Symbol: RBP3
• NCBI Official Synonym Symbols: IRBP; RBPI; RP66; D10S64; D10S65; D10S66
• NCBI Protein Information: retinol-binding protein 3
• UniProt Protein Name: Retinol-binding protein 3
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP3
• UniProt Synonym Gene Names: IRBP
• UniProt Entry Name: RET3_HUMAN

NCBI Description

Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively. [provided by RefSeq, Jul 2008]

Uniprot Description

RBP3: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. Belongs to the peptidase S41A family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; extracellular region; vesicle

Molecular Function: retinoid binding; serine-type peptidase activity; retinol binding; retinal binding

Biological Process: phototransduction, visible light; visual perception; transport; lipid metabolic process; proteolysis; retinoid metabolic process

Disease: Retinitis Pigmentosa 66; Retinitis Pigmentosa