INSIG1 sirna

INSIG1 siRNA (Rat)

Cat. Num. AAA8229420

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: INSIG1; INSIG1 siRNA (Rat); CL-6; Insulin-induced gene 1 protein; INSIG-1; Immediate-early protein CL-6; Insulin-induced growth response protein CL-6; INSIG1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: INSIG1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 259

• Applicable Applications for INSIG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat INSIG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for INSIG1 sirna

siRNA to inhibit INSIG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11693162
• NCBI GeneID: 64194
• NCBI Accession #: NP_071787.1
• NCBI GenBank Nucleotide #: NM_022392.1
• UniProt Accession #: Q08755
• Molecular Weight: 28,232 Da
• NCBI Official Full Name: insulin-induced gene 1 protein
• NCBI Official Synonym Full Names: insulin induced gene 1
• NCBI Official Symbol: Insig1
• NCBI Protein Information: insulin-induced gene 1 protein
• UniProt Protein Name: Insulin-induced gene 1 protein
• Protein Family: Insulin-induced gene 1 protein
• UniProt Gene Name: Insig1
• UniProt Synonym Gene Names: Cl-6; INSIG-1
• UniProt Entry Name: INSI1_RAT

NCBI Description

insulin-induced growth response gene; may play a role in regulating intracellular cholesterol concentrations [RGD, Feb 2006]

Uniprot Description

Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth ().