Specificity
ERCC5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Applicable Applications for ERCC5 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ERCC5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for ERCC5 sirna
siRNA to inhibit ERCC5 expression using RNA interference
NCBI and Uniprot Product Information
NCBI GenBank Nucleotide #
Molecular Weight
27,259 Da
NCBI Official Full Name
DNA repair protein complementing XP-G cells
NCBI Official Synonym Full Names
excision repair cross-complementation group 5
NCBI Official Symbol
ERCC5
NCBI Official Synonym Symbols
XPG; UVDR; XPGC; COFS3; ERCM2; ERCC5-201
NCBI Protein Information
DNA repair protein complementing XP-G cells
UniProt Protein Name
DNA repair protein complementing XP-G cells
UniProt Synonym Gene Names
ERCM2; XPG; XPGC
UniProt Entry Name
ERCC5_HUMAN
NCBI Description
This gene encodes a single-strand specific DNA endonuclease that makes the 3' incision in DNA excision repair following UV-induced damage. The protein may also function in other cellular processes, including RNA polymerase II transcription, and transcription-coupled DNA repair. Mutations in this gene cause xeroderma pigmentosum complementation group G (XP-G), which is also referred to as xeroderma pigmentosum VII (XP7), a skin disorder characterized by hypersensitivity to UV light and increased susceptibility for skin cancer development following UV exposure. Some patients also develop Cockayne syndrome, which is characterized by severe growth defects, mental retardation, and cachexia. Read-through transcription exists between this gene and the neighboring upstream BIVM (basic, immunoglobulin-like variable motif containing) gene. [provided by RefSeq, Feb 2011]
Uniprot Description
XPG: Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too. Defects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G); also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities. Belongs to the XPG/RAD2 endonuclease family. XPG subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; Deoxyribonuclease; EC 3.1.-.-
Chromosomal Location of Human Ortholog: 13q33
Cellular Component: nucleoplasm; intermediate filament cytoskeleton; DNA replication factor A complex; holo TFIIH complex; DNA-directed RNA polymerase II, holoenzyme; nucleus
Molecular Function: protein binding; protein homodimerization activity; endonuclease activity; metal ion binding; double-stranded DNA binding; protein N-terminus binding; bubble DNA binding; single-stranded DNA binding; endodeoxyribonuclease activity
Biological Process: nucleotide-excision repair, DNA incision, 3'-to lesion; UV protection; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; DNA repair; DNA catabolic process, endonucleolytic; response to UV-C; negative regulation of apoptosis; response to UV
Disease: Xeroderma Pigmentosum, Complementation Group G
Research Articles on ERCC5
Similar Products
Product Notes
The ERCC5 ercc5 (Catalog #
AAA8229344) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The ERCC5 siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC5 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the ERCC5 ercc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"ERCC5, siRNA" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech
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responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any
aspect of this datasheet at
any time and without notice. It is the responsibility of the customer to inform AAA Biotech
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