CHMP4B sirna

CHMP4B siRNA (Mouse)

Cat. Num. AAA8228915

• Gene Names: Chmp4b; C76846; Snf7-2; 2010012F05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHMP4B; CHMP4B siRNA (Mouse); Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; CHMP4b; CHMP4B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHMP4B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 224

• Applicable Applications for CHMP4B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHMP4B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHMP4B sirna

siRNA to inhibit CHMP4B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28077049
• NCBI GeneID: 75608
• NCBI Accession #: NP_083638.1
• NCBI GenBank Nucleotide #: NM_029362.3
• UniProt Accession #: Q9D8B3; Q3TXM7; Q91VM7; Q922P1; A2AVM1
• Molecular Weight: 24,936 Da
• NCBI Official Full Name: charged multivesicular body protein 4b
• NCBI Official Synonym Full Names: charged multivesicular body protein 4B
• NCBI Official Symbol: Chmp4b
• NCBI Official Synonym Symbols: C76846; Snf7-2; 2010012F05Rik
• NCBI Protein Information: charged multivesicular body protein 4b
• UniProt Protein Name: Charged multivesicular body protein 4b
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: Chmp4b
• UniProt Synonym Gene Names: CHMP4b
• UniProt Entry Name: CHM4B_MOUSE

Uniprot Description

CHMP4B: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. Defects in CHMP4B are the cause of cataract posterior polar type 3 (CTPP3). A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Belongs to the SNF7 family.

Protein type: Membrane protein, peripheral

Cellular Component: internal side of plasma membrane; membrane coat; membrane; cytoplasm; midbody; nucleus; endosome

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: protein transport; cell separation during cytokinesis; transport; vacuolar transport; non-lytic virus budding; regulation of viral reproduction; posttranslational protein targeting to membrane; mitotic metaphase plate congression; protein homooligomerization; nuclear organization and biogenesis