Western Blot (WB)
(Western blot analysis of GLURD2 expression in HEK293T (A), mouse brain (B) whole cell lysates.)
Rabbit GLURD2 Polyclonal Antibody | anti-GRID2 antibody
Anti-GLURD2 Antibody
Western Blot (WB)
(Western blot analysis of GLURD2 expression in HEK293T (A), mouse brain (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GLURD2 expression in HEK293T (A), mouse brain (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of GLURD2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of GLURD2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]
Uniprot Description
GluR-delta2: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.
Protein type: Channel, calcium; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated
Chromosomal Location of Human Ortholog: 4q22
Cellular Component: postsynaptic membrane; integral to plasma membrane; dendrite; plasma membrane; dendritic spine; ionotropic glutamate receptor complex; synapse; cell junction
Molecular Function: extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; glutamate receptor activity; PDZ domain binding
Biological Process: heterophilic cell adhesion; synaptic transmission, glutamatergic; prepulse inhibition; transport; glutamate signaling pathway; ionotropic glutamate receptor signaling pathway; regulation of neuron apoptosis; regulation of excitatory postsynaptic membrane potential; cerebellar granule cell differentiation
Disease: Spinocerebellar Ataxia, Autosomal Recessive 18
Research Articles on GRID2
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Product Notes
The GRID2 grid2 (Catalog #AAA8228496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GLURD2 Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's GLURD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB (1/500 - 1/1000), IF (1/100 - 1/500), ICC (1/100 - 1/500). Researchers should empirically determine the suitability of the GRID2 grid2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLURD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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