RAB39B sirna

RAB39B siRNA (Mouse)

Cat. Num. AAA8228245

• Gene Names: Rab39b; 6330580M05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAB39B; RAB39B siRNA (Mouse); Ras-related protein Rab-39B; RAB39B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAB39B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 213

• Applicable Applications for RAB39B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAB39B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAB39B sirna

siRNA to inhibit RAB39B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30424726
• NCBI GeneID: 67790
• NCBI Accession #: NP_780331.1
• NCBI GenBank Nucleotide #: NM_175122.6
• UniProt Accession #: Q8BHC1; Q3TUJ3
• Molecular Weight: 24,636 Da
• NCBI Official Full Name: ras-related protein Rab-39B
• NCBI Official Synonym Full Names: RAB39B, member RAS oncogene family
• NCBI Official Symbol: Rab39b
• NCBI Official Synonym Symbols: 6330580M05Rik
• NCBI Protein Information: ras-related protein Rab-39B
• UniProt Protein Name: Ras-related protein Rab-39B
• Protein Family: Ras-related protein
• UniProt Gene Name: Rab39b
• UniProt Entry Name: RB39B_MOUSE

NCBI Description

This gene encodes a member of the Ras-related small GTPases, which regulate membrane trafficking in organelles and transport vesicles. This protein has been reported to be enriched in mouse brain, and specifically within neurons, and may play a role in synapse formation. In humans mutations in this gene are associated with X-linked mental retardation. [provided by RefSeq, Jun 2013]

Uniprot Description

RAB39B: May be involved in vesicular trafficking. Plays a role in synapse formation. Defects in RAB39B are the cause of mental retardation X- linked type 72 (MRX72). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX72 patients can manifest autism spectrum disorder, seizures and macrocephaly as additional features. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Cellular Component: Golgi apparatus; neuron projection; membrane; cell; plasma membrane; intracellular; vesicle

Molecular Function: GTPase activity; myosin V binding; nucleotide binding

Biological Process: vesicle-mediated transport; intracellular protein transport; protein transport; transport; small GTPase mediated signal transduction; synapse organization and biogenesis; Rab protein signal transduction