VWA2 sirna

VWA2 siRNA (Human)

Cat. Num. AAA8227764

• Gene Names: VWA2; AMACO; NET42; CCSP-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VWA2; VWA2 siRNA (Human); AMACO; von Willebrand factor A domain-containing protein 2; A domain-containing protein similar to matrilin and collagen; Colon cancer secreted protein 2; CCSP-2; VWA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: VWA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 421

• Applicable Applications for VWA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human VWA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VWA2 sirna

siRNA to inhibit VWA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 440546401
• NCBI GeneID: 340706
• NCBI Accession #: NP_001258975.1
• NCBI GenBank Nucleotide #: NM_001272046.1
• UniProt Accession #: Q5GFL6; Q6ZS39; Q6ZWJ7; Q708C5; Q70UZ8; A1A5D4; B5MDJ8
• Molecular Weight: 45,345 Da
• NCBI Official Full Name: von Willebrand factor A domain-containing protein 2
• NCBI Official Synonym Full Names: von Willebrand factor A domain containing 2
• NCBI Official Symbol: VWA2
• NCBI Official Synonym Symbols: AMACO; NET42; CCSP-2
• NCBI Protein Information: von Willebrand factor A domain-containing protein 2
• UniProt Protein Name: von Willebrand factor A domain-containing protein 2
• Protein Family: von Willebrand factor A domain-containing protein
• UniProt Gene Name: VWA2
• UniProt Synonym Gene Names: AMACO; AMACO; CCSP-2
• UniProt Entry Name: VWA2_HUMAN

NCBI Description

This gene encodes a member of the von Willebrand factor A-like domain protein superfamily. The encoded protein is localized to the extracellular matrix and may serve as a structural component in basement membranes or in anchoring structures on scaffolds of collagen VII or fibrillin. This gene has been linked to type 1A diabetes and is a candidate serological marker for colon cancer. [provided by RefSeq, Jan 2013]

Uniprot Description

VWA2: 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: extracellular space; basement membrane

Molecular Function: identical protein binding

Biological Process: regulation of insulin receptor signaling pathway; calcium-independent cell-matrix adhesion; protein homooligomerization