COPG2 sirna

COPG2 siRNA (Mouse)

Cat. Num. AAA8227390

• Gene Names: Copg2; AW227625; gamma-2-COP
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COPG2; COPG2 siRNA (Mouse); Coatomer subunit gamma-2; Gamma-2-coat protein; Gamma-2-COP; COPG2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: COPG2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 870

• Applicable Applications for COPG2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COPG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COPG2 sirna

siRNA to inhibit COPG2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8567340
• NCBI GeneID: 54160
• NCBI Accession #: NP_059506.1
• NCBI GenBank Nucleotide #: NM_017478.3
• UniProt Accession #: Q9QXK3; Q3TQD5; Q3UEL6; Q8C2J6; A6H6J5
• Molecular Weight: 97,551 Da
• NCBI Official Full Name: coatomer subunit gamma-2
• NCBI Official Synonym Full Names: coatomer protein complex, subunit gamma 2
• NCBI Official Symbol: Copg2
• NCBI Official Synonym Symbols: AW227625; gamma-2-COP
• NCBI Protein Information: coatomer subunit gamma-2
• UniProt Protein Name: Coatomer subunit gamma-2
• Protein Family: Coatomer
• UniProt Gene Name: Copg2
• UniProt Synonym Gene Names: Gamma-2-COP
• UniProt Entry Name: COPG2_MOUSE

Uniprot Description

COPG2: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the COPG family.

Protein type: Motility/polarity/chemotaxis; Vesicle

Cellular Component: Golgi apparatus; membrane coat; membrane; cytoplasm; COPI vesicle coat; cytoplasmic vesicle

Molecular Function: structural molecule activity

Biological Process: vesicle-mediated transport; intra-Golgi vesicle-mediated transport; intracellular protein transport; protein transport; transport