SYT2 sirna

SYT2 siRNA (Rat)

Cat. Num. AAA8227024

• Gene Names: Syt2; SYNII; RATSYNII
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SYT2; SYT2 siRNA (Rat); Synaptotagmin-2; Synaptotagmin II; SytII; SYT2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SYT2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 422

• Applicable Applications for SYT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SYT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SYT2 sirna

siRNA to inhibit SYT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981624
• NCBI GeneID: 24805
• NCBI Accession #: NP_036797.1
• NCBI GenBank Nucleotide #: NM_012665.1
• UniProt Accession #: P29101
• Molecular Weight: 47,210 Da
• NCBI Official Full Name: synaptotagmin-2
• NCBI Official Synonym Full Names: synaptotagmin II
• NCBI Official Symbol: Syt2
• NCBI Official Synonym Symbols: SYNII; RATSYNII
• NCBI Protein Information: synaptotagmin-2
• UniProt Protein Name: Synaptotagmin-2
• Protein Family: Synaptotagmin
• UniProt Gene Name: Syt2
• UniProt Synonym Gene Names: SytII
• UniProt Entry Name: SYT2_RAT

NCBI Description

component of synaptic vesicle membranes; may have important role in vesicle trafficking to the active zone of the synapse and in exocytosis; may be the crucial calcium sensor in neurotransmitter release [RGD, Feb 2006]

Uniprot Description

SYT2: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. Belongs to the synaptotagmin family.

Protein type: Lipid-binding; Calcium-binding; Membrane protein, integral; Vesicle

Cellular Component: synaptic vesicle; synaptic vesicle membrane; intracellular membrane-bound organelle; plasma membrane; integral to membrane; terminal button; cell junction

Molecular Function: protein binding; syntaxin binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding

Biological Process: synaptic vesicle exocytosis; transport