DLL3 sirna

DLL3 siRNA (Human)

Cat. Num. AAA8226973

• Gene Names: DLL3; SCDO1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLL3; DLL3 siRNA (Human); Delta-like protein 3; Drosophila Delta homolog 3; Delta3; DLL3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DLL3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 587

• Applicable Applications for DLL3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DLL3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLL3 sirna

siRNA to inhibit DLL3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8393264
• NCBI GeneID: 10683
• NCBI Accession #: NP_058637.1
• NCBI GenBank Nucleotide #: NM_016941.3
• UniProt Accession #: Q9NYJ7; Q8NBS4; E9PFG2
• Molecular Weight: 61,178 Da
• NCBI Official Full Name: delta-like protein 3 isoform 1
• NCBI Official Synonym Full Names: delta-like 3 (Drosophila)
• NCBI Official Symbol: DLL3
• NCBI Official Synonym Symbols: SCDO1
• NCBI Protein Information: delta-like protein 3
• UniProt Protein Name: Delta-like protein 3
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL3
• UniProt Synonym Gene Names: Delta3
• UniProt Entry Name: DLL3_HUMAN

NCBI Description

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor.

Protein type: Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: integral to membrane

Molecular Function: Notch binding

Biological Process: paraxial mesoderm development; compartment specification; somitogenesis; Notch signaling pathway; skeletal development; negative regulation of neurogenesis

Disease: Spondylocostal Dysostosis 1, Autosomal Recessive