MAP3K11 sirna

MAP3K11 siRNA (Rat)

Cat. Num. AAA8226251

• Gene Names: Map3k11; Mlk3; RHOE; Mlk-3
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAP3K11; MAP3K11 siRNA (Rat); MLK3; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MAP3K11 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MAP3K11 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 850

• Applicable Applications for MAP3K11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MAP3K11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAP3K11 sirna

siRNA to inhibit MAP3K11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61557106
• NCBI GeneID: 309168
• NCBI Accession #: NP_001013168.1
• NCBI GenBank Nucleotide #: NM_001013150.1
• UniProt Accession #: Q66HA1; Q80YF1
• Molecular Weight: 93,109 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Symbol: Map3k11
• NCBI Official Synonym Symbols: Mlk3; RHOE; Mlk-3
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 11
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 11
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: Map3k11
• UniProt Synonym Gene Names: Mlk3
• UniProt Entry Name: M3K11_RAT

Uniprot Description

MLK3: Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle (By similarity)

Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; TKL group; MLK family; MLK subfamily

Cellular Component: microtubule; centrosome; membrane; cytoplasm

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; protein homodimerization activity; JUN kinase kinase kinase activity; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein-tyrosine kinase activity; Rac GTPase binding; mitogen-activated protein kinase kinase kinase binding; ATP binding

Biological Process: cell death; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; activation of MAPK activity; positive regulation of apoptosis; protein amino acid autophosphorylation; positive regulation of JNK cascade; protein amino acid phosphorylation; activation of JNK activity; microtubule-based process; cell proliferation; positive regulation of neuron apoptosis; JNK cascade; activation of JNKK activity