DEGS1 sirna

DEGS1 siRNA (Human)

Cat. Num. AAA8226166

• Gene Names: DEGS1; MLD; DEGS; DES1; Des-1; FADS7; MIG15; DEGS-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DEGS1; DEGS1 siRNA (Human); DES1; MLD; Sphingolipid delta(4)-desaturase DES1; Cell migration-inducing gene 15 protein; Degenerative spermatocyte homolog 1; Membrane lipid desaturase; DEGS1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DEGS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for DEGS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DEGS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DEGS1 sirna

siRNA to inhibit DEGS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505193
• NCBI GeneID: 8560
• NCBI Accession #: NP_003667.1
• NCBI GenBank Nucleotide #: NM_003676.3
• UniProt Accession #: O15121
• Molecular Weight: 37,866 Da
• NCBI Official Full Name: sphingolipid delta(4)-desaturase DES1
• NCBI Official Synonym Full Names: delta(4)-desaturase, sphingolipid 1
• NCBI Official Symbol: DEGS1
• NCBI Official Synonym Symbols: MLD; DEGS; DES1; Des-1; FADS7; MIG15; DEGS-1
• NCBI Protein Information: sphingolipid delta(4)-desaturase DES1
• UniProt Protein Name: Sphingolipid delta(4)-desaturase DES1
• UniProt Gene Name: DEGS1
• UniProt Synonym Gene Names: DES1; MLD
• UniProt Entry Name: DEGS1_HUMAN

NCBI Description

This gene encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. [provided by RefSeq, Mar 2010]

Uniprot Description

DEGS1: Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). Belongs to the fatty acid desaturase family. DEGS subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Lipid Metabolism - sphingolipid; EC 1.14.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q42.11

Cellular Component: endoplasmic reticulum membrane; mitochondrion; membrane; endoplasmic reticulum; integral to plasma membrane

Molecular Function: electron carrier activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; unsaturated fatty acid biosynthetic process