SH3TC2 sirna

SH3TC2 siRNA (Human)

Cat. Num. AAA8225915

• Gene Names: SH3TC2; MNMN; CMT4C
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SH3TC2; SH3TC2 siRNA (Human); KIAA1985; SH3 domain and tetratricopeptide repeat-containing protein 2; SH3TC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SH3TC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,281

• Applicable Applications for SH3TC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SH3TC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SH3TC2 sirna

siRNA to inhibit SH3TC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38488692
• NCBI GeneID: 79628
• NCBI Accession #: NP_078853.2
• NCBI GenBank Nucleotide #: NM_024577.3
• UniProt Accession #: Q8TF17; Q14CC0; Q14CF5; Q9H8I5; B3KWE5
• Molecular Weight: 144,023 Da
• NCBI Official Full Name: SH3 domain and tetratricopeptide repeat-containing protein 2
• NCBI Official Synonym Full Names: SH3 domain and tetratricopeptide repeats 2
• NCBI Official Symbol: SH3TC2
• NCBI Official Synonym Symbols: MNMN; CMT4C
• NCBI Protein Information: SH3 domain and tetratricopeptide repeat-containing protein 2
• UniProt Protein Name: SH3 domain and tetratricopeptide repeat-containing protein 2
• Protein Family: SH3 domain and tetratricopeptide repeat-containing protein
• UniProt Gene Name: SH3TC2
• UniProt Synonym Gene Names: KIAA1985
• UniProt Entry Name: S3TC2_HUMAN

NCBI Description

This gene encodes a protein with two N-terminal Src homology 3 (SH3) domains and 10 tetratricopeptide repeat (TPR) motifs, and is a member of a small gene family. The gene product has been proposed to be an adapter or docking molecule. Mutations in this gene result in autosomal recessive Charcot-Marie-Tooth disease type 4C, a childhood-onset neurodegenerative disease characterized by demyelination of motor and sensory neurons. [provided by RefSeq, Jul 2008]

Uniprot Description

SH3TC2: Defects in SH3TC2 are the cause of Charcot-Marie-Tooth disease type 4C (CMT4C). CMT4C is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot- Marie-Tooth disease are designated CMT4. CMT4C is characterized by onset in childhood, early-onset scoliosis and a distinct Schwann cell pathology. Defects in SH3TC2 are the cause of mononeuropathy of the median nerve mild (MNMN). A disease characterized by median nerve mononeuropathy at the wrist. The clinical presentation ranges from a mild phenotype, consistent with carpal tunnel syndrome, to a severe median nerve mononeuropathy at the wrist associated with evidence of a more widespread axonal polyneuropathy. The latter phenotype is similar to that of patients with hereditary neuropathy with liability to pressure palsies. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: recycling endosome; plasma membrane; cytoplasmic vesicle

Biological Process: regulation of intracellular protein transport; myelin maintenance in the peripheral nervous system

Disease: Charcot-marie-tooth Disease, Type 4c