TMED9 sirna

TMED9 siRNA (Mouse)

Cat. Num. AAA8225847

• Gene Names: Tmed9; 2400003B06Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMED9; TMED9 siRNA (Mouse); GP25L2; Transmembrane emp24 domain-containing protein 9; Glycoprotein 25L2; p24 family protein alpha-2; p24alpha2; TMED9 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TMED9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 235

• Applicable Applications for TMED9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TMED9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMED9 sirna

siRNA to inhibit TMED9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 145966911
• NCBI GeneID: 67511
• NCBI Accession #: NP_080487.2
• NCBI GenBank Nucleotide #: NM_026211.3
• UniProt Accession #: Q99KF1; Q9CX02
• Molecular Weight: 27,127 Da
• NCBI Official Full Name: transmembrane emp24 domain-containing protein 9
• NCBI Official Synonym Full Names: transmembrane emp24 protein transport domain containing 9
• NCBI Official Symbol: Tmed9
• NCBI Official Synonym Symbols: 2400003B06Rik
• NCBI Protein Information: transmembrane emp24 domain-containing protein 9
• UniProt Protein Name: Transmembrane emp24 domain-containing protein 9
• Protein Family: Transmembrane emp24 domain-containing protein
• UniProt Gene Name: Tmed9
• UniProt Synonym Gene Names: Gp25l2; p24alpha2
• UniProt Entry Name: TMED9_MOUSE

Uniprot Description

TMED9: Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer- dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral

Cellular Component: Golgi membrane; Golgi apparatus; membrane; endoplasmic reticulum; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: syntaxin binding

Biological Process: protein transport; COPI coating of Golgi vesicle; transport; Golgi organization and biogenesis