CACNG8 sirna

CACNG8 siRNA (Rat)

Cat. Num. AAA8225745

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CACNG8; CACNG8 siRNA (Rat); Voltage-dependent calcium channel gamma-8 subunit; Neuronal voltage-gated calcium channel gamma-8 subunit; Transmembrane AMPAR regulatory protein gamma-8; TARP gamma-8; CACNG8 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CACNG8 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 421

• Applicable Applications for CACNG8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CACNG8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CACNG8 sirna

siRNA to inhibit CACNG8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21687018
• NCBI GeneID: 140729
• NCBI Accession #: NP_542427.1
• NCBI GenBank Nucleotide #: NM_080696.2
• UniProt Accession #: Q8VHW5
• Molecular Weight: 43,269 Da
• NCBI Official Full Name: voltage-dependent calcium channel gamma-8 subunit
• NCBI Official Synonym Full Names: calcium channel, voltage-dependent, gamma subunit 8
• NCBI Official Symbol: Cacng8
• NCBI Protein Information: voltage-dependent calcium channel gamma-8 subunit
• UniProt Protein Name: Voltage-dependent calcium channel gamma-8 subunit
• Protein Family: Voltage-dependent calcium channel
• UniProt Gene Name: Cacng8
• UniProt Synonym Gene Names: TARP gamma-8
• UniProt Entry Name: CCG8_RAT

NCBI Description

Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of the pore-forming alpha-1 subunit and the modulatory beta, alpha-2/delta and gamma subunits. The protein encoded by this gene represents one of the gamma subunits. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This gene is located in a cluster with two similar gamma subunit-encoding genes on chromosome 1. The mRNA for this gene is believed to initiate translation from a non-AUG (CUG) start codon. [provided by RefSeq, Jul 2008]

Uniprot Description

CACNG8: Regulates the trafficking and gating properties of AMPA- selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state. Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, calcium

Cellular Component: postsynaptic membrane; postsynaptic density; integral to membrane; cell junction

Molecular Function: protein binding; calcium channel activity; voltage-gated ion channel activity