ELL sirna

ELL siRNA (Human)

Cat. Num. AAA8225095

• Gene Names: ELL; MEN; ELL1; PPP1R68; C19orf17
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ELL; ELL siRNA (Human); C19orf17; RNA polymerase II elongation factor ELL; Eleven-nineteen lysine-rich leukemia protein; ELL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ELL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 621

• Applicable Applications for ELL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ELL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ELL sirna

siRNA to inhibit ELL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5729812
• NCBI GeneID: 8178
• NCBI Accession #: NP_006523.1
• NCBI GenBank Nucleotide #: NM_006532.3
• UniProt Accession #: P55199
• Molecular Weight: 68,265 Da
• NCBI Official Full Name: RNA polymerase II elongation factor ELL
• NCBI Official Synonym Full Names: elongation factor RNA polymerase II
• NCBI Official Symbol: ELL
• NCBI Official Synonym Symbols: MEN; ELL1; PPP1R68; C19orf17
• NCBI Protein Information: RNA polymerase II elongation factor ELL
• UniProt Protein Name: RNA polymerase II elongation factor ELL
• Protein Family: ELL-associated factor
• UniProt Gene Name: ELL
• UniProt Synonym Gene Names: C19orf17
• UniProt Entry Name: ELL_HUMAN

Uniprot Description

ELL: Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. A chromosomal aberration involving ELL is found in acute leukemias. Translocation t(11;19)(q23;p13.1) with MLL/HRX. The result is a rogue activator protein. Belongs to the ELL/occludin family.

Protein type: Nuclear receptor co-regulator; Transcription initiation complex; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; Cajal body; transcription elongation factor complex; nuclear speck

Molecular Function: protein binding; phosphatase binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of RNA elongation from RNA polymerase II promoter; in utero embryonic development; positive regulation of viral transcription; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; gene expression; snRNA transcription from RNA polymerase III promoter