Western Blot (WB)
(Western blot analysis of GCN5 expression in K562 (A), A431 (B), mouse brain (C) whole cell lysates.)
Rabbit GCN5 Polyclonal Antibody | anti-KAT2A antibody
Anti-GCN5 Antibody
Western Blot (WB)
(Western blot analysis of GCN5 expression in K562 (A), A431 (B), mouse brain (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GCN5 expression in K562 (A), A431 (B), mouse brain (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GCN5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GCN5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009]
Uniprot Description
GCN5: an ubiquitous histone acetyltransferase that promotes transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. Interacts with P300, CBP, ADA2, and is a component of the TFTC-HAT complex. Two splice variant isoforms have been described.
Protein type: EC 2.3.1.48; Acetyltransferase
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: nucleoplasm; extracellular space
Molecular Function: protein binding; histone acetyltransferase activity; histone deacetylase binding; transcription coactivator activity; chromatin binding; H3 histone acetyltransferase activity
Biological Process: transcription from RNA polymerase II promoter; somitogenesis; establishment and/or maintenance of chromatin architecture; viral reproduction; in utero embryonic development; multicellular organism growth; regulation of protein stability; metencephalon development; histone deubiquitination; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; cell proliferation; telencephalon development; midbrain development; neural tube closure
Research Articles on KAT2A
Similar Products
Product Notes
The KAT2A kat2a (Catalog #AAA8224919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GCN5 Antibody reacts with Human, Mouse, Rat, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's GCN5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the KAT2A kat2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCN5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
