MED12 sirna

MED12 siRNA (Mouse)

Cat. Num. AAA8224891

• Gene Names: Med12; Mopa; OPA-1; 230kDa; Tnrc11; Trap230
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MED12; MED12 siRNA (Mouse); KIAA0192; MOPA; TNRC11; TRAP230; Mediator of RNA polymerase II transcription subunit 12; Mediator complex subunit 12; OPA-containing protein; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Trinucleotide repeat-containing gene 11 protein; MED12 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MED12 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2190

• Applicable Applications for MED12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MED12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MED12 sirna

siRNA to inhibit MED12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 125628662
• NCBI GeneID: 59024
• NCBI Accession #: NP_067496.2
• NCBI GenBank Nucleotide #: NM_021521.2
• UniProt Accession #: A2AGH6; O88542; Q571H3; Q6PGB4; Q6PGD8; A2AGH7
• Molecular Weight: 97,885 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 12
• NCBI Official Synonym Full Names: mediator complex subunit 12
• NCBI Official Symbol: Med12
• NCBI Official Synonym Symbols: Mopa; OPA-1; 230kDa; Tnrc11; Trap230
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 12
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 12
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: Med12
• UniProt Synonym Gene Names: Kiaa0192; Mopa; Tnrc11; Trap230; Trap230
• UniProt Entry Name: MED12_MOUSE

Uniprot Description

MED12: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; centrosome; membrane; nucleolus; Srb-mediator complex; nucleus; ubiquitin ligase complex

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; transcription coactivator activity; transcription cofactor activity; beta-catenin binding; chromatin binding; thyroid hormone receptor binding; receptor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Schwann cell development; negative regulation of Wnt receptor signaling pathway; transcription, DNA-dependent; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of transcription, DNA-dependent; heart development; stem cell maintenance; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; neural tube closure; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; oligodendrocyte development