SVIL sirna

SVIL siRNA (Human)

Cat. Num. AAA8224362

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SVIL; SVIL siRNA (Human); Supervillin; Archvillin; p205/p250; SVIL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SVIL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1788

• Applicable Applications for SVIL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SVIL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SVIL sirna

siRNA to inhibit SVIL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 150417971
• NCBI GeneID: 6840
• NCBI Accession #: NP_003165.2
• NCBI GenBank Nucleotide #: NM_003174.3
• UniProt Accession #: O95425; O60611; O60612; Q5VZK5; Q5VZK6; Q9H1R7; D3DRW9; M1J557
• Molecular Weight: 244,523 Da
• NCBI Official Full Name: supervillin isoform 1
• NCBI Official Synonym Full Names: supervillin
• NCBI Official Symbol: SVIL
• NCBI Protein Information: supervillin
• UniProt Protein Name: Supervillin
• Protein Family: Supervillin
• UniProt Gene Name: SVIL
• UniProt Entry Name: SVIL_HUMAN

NCBI Description

This gene encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. The gene product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Two transcript variants encoding different isoforms of supervillin have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

supervillin: an actin-binding protein that links filamentous actin to the plasma membrane. Binds filamentous actin and myosin II bind within the rsds 1-830 of the amino terminus, an extended intrinsically disordered region (IDR). Forms a high-affinity link between the actin cytoskeleton and the plasma membrane. Four human isoforms are formed by alternative splicing. Isoform 1 (archvillin) is muscle specific and among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Contractile; Actin-binding

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: costamere; focal adhesion; cytoplasm; plasma membrane; podosome; midbody; nucleus; cleavage furrow; actin cytoskeleton

Molecular Function: actin filament binding; protein binding

Biological Process: positive regulation of cytokinesis; skeletal muscle development; cytoskeleton organization and biogenesis