PFKP sirna

PFKP siRNA (Human)

Cat. Num. AAA8224148

• Gene Names: PFKP; PFKF; PFK-C; PFK-P; ATP-PFK
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PFKP; PFKP siRNA (Human); PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase. platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase; PFKP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PFKP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 776

• Applicable Applications for PFKP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PFKP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PFKP sirna

siRNA to inhibit PFKP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 334191701
• NCBI GeneID: 5214
• NCBI Accession #: NP_001229268.1
• NCBI GenBank Nucleotide #: NM_001242339.1
• UniProt Accession #: Q01813; Q5VSR7; Q5VSR8; B3KS15
• Molecular Weight: 85,316 Da
• NCBI Official Full Name: ATP-dependent 6-phosphofructokinase, platelet type isoform 2
• NCBI Official Synonym Full Names: phosphofructokinase, platelet
• NCBI Official Symbol: PFKP
• NCBI Official Synonym Symbols: PFKF; PFK-C; PFK-P; ATP-PFK
• NCBI Protein Information: ATP-dependent 6-phosphofructokinase, platelet type
• UniProt Protein Name: ATP-dependent 6-phosphofructokinase, platelet type
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKP
• UniProt Synonym Gene Names: PFKF; ATP-PFK; PFK-P
• UniProt Entry Name: PFKAP_HUMAN

NCBI Description

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008]

Uniprot Description

PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose; Kinase, other

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: membrane; nucleus; cytosol

Molecular Function: metal ion binding; protein complex binding; ATP binding; 6-phosphofructokinase activity

Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process