SMAD2 sirna

SMAD2 siRNA (Rat)

Cat. Num. AAA8223081

• Gene Names: Smad2; Madh2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMAD2; SMAD2 siRNA (Rat); MADH2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; Mad-related protein 2; SMAD family member 2; SMAD 2; Smad2; SMAD2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SMAD2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 467

• Applicable Applications for SMAD2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SMAD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMAD2 sirna

siRNA to inhibit SMAD2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 477507190
• NCBI GeneID: 29357
• NCBI Accession #: NP_001264379.1
• NCBI GenBank Nucleotide #: NM_001277450.1
• UniProt Accession #: O70436; A0JPM1
• Molecular Weight: 52,239 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 2
• NCBI Official Synonym Full Names: SMAD family member 2
• NCBI Official Symbol: Smad2
• NCBI Official Synonym Symbols: Madh2
• NCBI Protein Information: mothers against decapentaplegic homolog 2
• UniProt Protein Name: Mothers against decapentaplegic homolog 2
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: Smad2
• UniProt Synonym Gene Names: Madh2; MAD homolog 2; Mothers against DPP homolog 2; SMAD 2; Smad2
• UniProt Entry Name: SMAD2_RAT

NCBI Description

transcriptional mediator for both activin and TGFbeta [RGD, Feb 2006]

Uniprot Description

SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Cellular Component: transcription factor complex; protein complex; cell; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein homodimerization activity; transcription activator binding; metal ion binding; transcription factor binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; protein binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transforming growth factor beta receptor binding; transcription factor activity

Biological Process: developmental growth; positive regulation of transcription, DNA-dependent; heart development; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; palate development; embryonic pattern specification; protein amino acid phosphorylation; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; pancreas development; response to glucose stimulus; positive regulation of BMP signaling pathway; pericardium development; cell fate commitment; transcription, DNA-dependent; regulation of binding; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; pattern specification process; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; endoderm formation; insulin secretion; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; lung development; endoderm development