Rabbit anti-Human CDCA3 Polyclonal Antibody | anti-CDCA3 antibody

Anti-CDCA3 Antibody

Cat. Num. AAA822302

• Gene Names: CDCA3; GRCC8; TOME-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: CDCA3; Polyclonal Antibody; Anti-CDCA3 Antibody; C8; GRCC8; TOME1; Cell division cycle-associated protein 3; Gene-rich cluster protein C8; Trigger of mitotic entry protein 1; TOME-1; anti-CDCA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of CDCA3 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 243

• Applicable Applications for anti-CDCA3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CDCA3. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of CDCA3 expression in MCF7 (A), Caco2 (B), HepG2 (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of CDCA3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunofluorescence (IF)

(Immunofluorescent analysis of CDCA3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

Related Product Information for anti-CDCA3 antibody

Rabbit polyclonal antibody to CDCA3

NCBI and Uniprot Product Information

• NCBI GI #: 662236832
• NCBI GeneID: 83461
• NCBI Accession #: NP_001284531.1
• NCBI GenBank Nucleotide #: NM_001297602.1
• UniProt Accession #: Q99618; A8K5V6; D3DUS6
• Molecular Weight: 28,998 Da
• NCBI Official Full Name: cell division cycle-associated protein 3 isoform 2
• NCBI Official Synonym Full Names: cell division cycle associated 3
• NCBI Official Symbol: CDCA3
• NCBI Official Synonym Symbols: GRCC8; TOME-1
• NCBI Protein Information: cell division cycle-associated protein 3; gene rich cluster, C8; trigger of mitotic entry 1; gene-rich cluster protein C8; trigger of mitotic entry protein 1
• UniProt Protein Name: Cell division cycle-associated protein 3
• Protein Family: Cell division cycle-associated protein
• UniProt Gene Name: CDCA3
• UniProt Synonym Gene Names: C8; GRCC8; TOME1; TOME-1
• UniProt Entry Name: CDCA3_HUMAN

Uniprot Description

CDCA3: F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytosol

Biological Process: mitosis; cell division; protein ubiquitination