CIITA sirna

CIITA siRNA (Mouse)

Cat. Num. AAA8222821

• Gene Names: Ciita; C2ta; Gm9475; Mhc2ta; EG669998
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CIITA; CIITA siRNA (Mouse); C2TA; MHC2TA; MHC class II transactivator; CIITA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CIITA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1075

• Applicable Applications for CIITA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CIITA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CIITA sirna

siRNA to inhibit CIITA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 344217728
• NCBI GeneID: 12265
• NCBI Accession #: NP_001230690.1
• NCBI GenBank Nucleotide #: NM_001243761.2
• UniProt Accession #: P79621; O46787; O78036; O78109; Q31115; Q9TPP1
• Molecular Weight: 116,233 Da
• NCBI Official Full Name: MHC class II transactivator isoform 3
• NCBI Official Synonym Full Names: class II transactivator
• NCBI Official Symbol: Ciita
• NCBI Official Synonym Symbols: C2ta; Gm9475; Mhc2ta; EG669998
• NCBI Protein Information: MHC class II transactivator
• UniProt Protein Name: MHC class II transactivator
• Protein Family: MHC class II transactivator
• UniProt Gene Name: Ciita
• UniProt Synonym Gene Names: C2ta; Mhc2ta; CIITA
• UniProt Entry Name: C2TA_MOUSE

NCBI Description

This gene encodes a member of the NOD-like receptor protein family. This protein acts as a transcriptional coactivator and component of the enhanceosome complex to stimulate transcription of MHC class II genes in the adaptive immune response. This protein may also regulate the transcription of MHC class I genes. Mutations in the human gene have been linked to a rare immunodeficiency, bare lymphocyte syndrome, and homozygous knockout mice exhibit many features of this disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

CIITA: a non-DNA binding transactivator that functions both in constitutive and inducible MHC Class II expression. Defects are a cause of bare lymphocyte syndrome type II (BLS II), a form of severe combined immunodeficiency disease (SCID) characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, an absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production.

Protein type: EC 2.7.11.1; DNA-binding; Transcription, coactivator/corepressor

Cellular Component: PML body; cell surface; nucleus

Molecular Function: glucosaminyl-phosphotidylinositol O-acyltransferase activity; S-malonyltransferase activity; S-acetyltransferase activity; O-octanoyltransferase activity; O-sinapoyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; Ras palmitoyltransferase activity; palmitoyltransferase activity; protein complex binding; C-palmitoyltransferase activity; ATP binding; malonyltransferase activity; octanoyltransferase activity; O-succinyltransferase activity; sterol O-acyltransferase activity; nucleotide binding; O-acyltransferase activity; O-acetyltransferase activity; benzoyl acetate-CoA thiolase activity; transferase activity; sinapoyltransferase activity; S-acyltransferase activity; N-succinyltransferase activity; L-2-aminoadipate N-acetyltransferase activity; keto acid formate lyase activity; GTP binding; 3-hydroxybutyryl-CoA thiolase activity; acyl-CoA N-acyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; succinyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; C-acyltransferase activity; dihydrolipoamide S-acyltransferase activity; protein C-terminus binding; N-acetyltransferase activity; protein-cysteine S-acyltransferase activity; serine O-acyltransferase activity; O-palmitoyltransferase activity; transcription activator binding; 3-ketopimelyl-CoA thiolase activity; carnitine O-acyltransferase activity; S-succinyltransferase activity; N-acyltransferase activity; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity; N-palmitoyltransferase activity; acylglycerol O-acyltransferase activity; protein-cysteine S-myristoyltransferase activity; acetyltransferase activity; myristoyltransferase activity; transferase activity, transferring acyl groups; kinase activity

Biological Process: response to antibiotic; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of MHC class I biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; phosphorylation