SPI1 sirna

SPI1 siRNA (Mouse)

Cat. Num. AAA8221698

• Gene Names: Spi1; Dis1; PU.1; Dis-1; Sfpi1; Spi-1; Sfpi-1; Tcfpu1; Tfpu.1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPI1; SPI1 siRNA (Mouse); SFPI-1; SFPI1; Transcription factor PU.1; 31 kDa-transforming protein; SFFV proviral integration 1 protein; SPI1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SPI1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 272

• Applicable Applications for SPI1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SPI1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPI1 sirna

siRNA to inhibit SPI1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755474
• NCBI GeneID: 20375
• NCBI Accession #: NP_035485.1
• NCBI GenBank Nucleotide #: NM_011355.1
• UniProt Accession #: P17433; Q99L57
• Molecular Weight: 31,349 Da
• NCBI Official Full Name: transcription factor PU.1
• NCBI Official Synonym Full Names: spleen focus forming virus (SFFV) proviral integration oncogene
• NCBI Official Symbol: Spi1
• NCBI Official Synonym Symbols: Dis1; PU.1; Dis-1; Sfpi1; Spi-1; Sfpi-1; Tcfpu1; Tfpu.1
• NCBI Protein Information: transcription factor PU.1
• UniProt Protein Name: Transcription factor PU.1
• Protein Family: Transcription factor
• UniProt Gene Name: Spi1
• UniProt Synonym Gene Names: Sfpi-1; Sfpi1
• UniProt Entry Name: SPI1_MOUSE

Uniprot Description

PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Oncoprotein

Cellular Component: transcription factor complex; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; DNA binding; RNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; granulocyte differentiation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; myeloid dendritic cell differentiation; lymphoid progenitor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; anatomical structure regression; regulation of erythrocyte differentiation; regulation of transcription from RNA polymerase II promoter; vasculature development; regulation of transcription, DNA-dependent; lymphocyte differentiation; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; myeloid leukocyte differentiation; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent