MINK1 sirna

MINK1 siRNA (Human)

Cat. Num. AAA8221672

• Gene Names: MINK1; B55; ZC3; MINK; YSK2; hMINK; MAP4K6; hMINKbeta
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MINK1; MINK1 siRNA (Human); B55; MAP4K6; MINK; YSK2; ZC3; Misshapen-like kinase 1; GCK family kinase MiNK; MAPK/ERK kinase kinase kinase 6; MEK kinase kinase 6; MEKKK 6; Misshapen/NIK-related kinase; Mitogen-activated protein kinase kinase kinase kinase 6; MINK1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MINK1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1312

• Applicable Applications for MINK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MINK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MINK1 sirna

siRNA to inhibit MINK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 68161546
• NCBI GeneID: 50488
• NCBI Accession #: NP_001020108.1
• NCBI GenBank Nucleotide #: NM_001024937.3
• UniProt Accession #: Q8N4C8; Q5U8Z0; Q9P1X1; Q9P2R8; D3DTK3; D3DTK4
• Molecular Weight: 143,637 Da
• NCBI Official Full Name: misshapen-like kinase 1 isoform 4
• NCBI Official Synonym Full Names: misshapen-like kinase 1
• NCBI Official Symbol: MINK1
• NCBI Official Synonym Symbols: B55; ZC3; MINK; YSK2; hMINK; MAP4K6; hMINKbeta
• NCBI Protein Information: misshapen-like kinase 1
• UniProt Protein Name: Misshapen-like kinase 1
• Protein Family: Misshapen-like kinase
• UniProt Gene Name: MINK1
• UniProt Synonym Gene Names: MEK kinase kinase 6; MEKKK 6
• UniProt Entry Name: MINK1_HUMAN

NCBI Description

This gene encodes a serine/threonine kinase belonging to the germinal center kinase (GCK) family. The protein is structurally similar to the kinases that are related to NIK and may belong to a distinct subfamily of NIK-related kinases within the GCK family. Studies of the mouse homolog indicate an up-regulation of expression in the course of postnatal mouse cerebral development and activation of the cJun N-terminal kinase (JNK) and the p38 pathways. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

MINK: a protein kinase of the STE20 family. Studies of the mouse ortholog indicate increased expression during postnatal mouse cerebral development. Activates JNK and the p38 pathways. Three splicing isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, STE; Kinase, protein; STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: Golgi apparatus; postsynaptic membrane; axon; dendrite; postsynaptic density; cytoplasm; cytosol; cell junction

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: regulation of cell-matrix adhesion; protein amino acid autophosphorylation; multicellular organismal development; dendrite morphogenesis; positive regulation of JNK cascade; regulation of cell migration; negative thymic T cell selection; protein amino acid phosphorylation; microvillus biogenesis; synaptic transmission; regulation of cell-cell adhesion; regulation of catalytic activity; actin cytoskeleton reorganization; JNK cascade; response to stress