AKT1 sirna

AKT1 siRNA (Mouse)

Cat. Num. AAA8221478

• Gene Names: Akt1; Akt; PKB; Rac; PKB/Akt; PKBalpha
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AKT1; AKT1 siRNA (Mouse); AKT; RAC; RAC-alpha serine/threonine-protein kinase; AKT1 kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; Thymoma viral proto-oncogene; AKT1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AKT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 488

• Applicable Applications for AKT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AKT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AKT1 sirna

siRNA to inhibit AKT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 260166608
• NCBI GeneID: 11651
• NCBI Accession #: NP_001159366.1
• NCBI GenBank Nucleotide #: NM_001165894.1
• UniProt Accession #: P31750; Q62274; Q6GSA6
• Molecular Weight: 55,707 Da
• NCBI Official Full Name: RAC-alpha serine/threonine-protein kinase isoform 2
• NCBI Official Synonym Full Names: thymoma viral proto-oncogene 1
• NCBI Official Symbol: Akt1
• NCBI Official Synonym Symbols: Akt; PKB; Rac; PKB/Akt; PKBalpha
• NCBI Protein Information: RAC-alpha serine/threonine-protein kinase
• UniProt Protein Name: RAC-alpha serine/threonine-protein kinase
• Protein Family: Potassium channel
• UniProt Gene Name: Akt1
• UniProt Synonym Gene Names: Akt; Rac; PKB; PKB alpha
• UniProt Entry Name: AKT1_MOUSE

NCBI Description

This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Oncoprotein; Protein kinase, AGC; AGC group; AKT family

Cellular Component: nucleoplasm; microtubule cytoskeleton; protein complex; membrane; mitochondrion; cytoplasm; plasma membrane; spindle; intracellular; intercellular junction; cytosol; nucleus

Molecular Function: identical protein binding; protein phosphatase 2A binding; nitric-oxide synthase regulator activity; nucleotide binding; protein kinase binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; enzyme binding; protein kinase C binding; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding

Biological Process: glycogen metabolic process; negative regulation of JNK cascade; positive regulation of nitric oxide biosynthetic process; regulation of myelination; positive regulation of apoptosis; multicellular organismal development; protein ubiquitination; glucose homeostasis; regulation of cell migration; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; germ cell development; positive regulation of glucose import; regulation of protein localization; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; nervous system development; glycogen biosynthetic process; positive regulation of nitric-oxide synthase activity; carbohydrate transport; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; carbohydrate metabolic process; negative regulation of protein kinase activity; striated muscle cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of transcription factor activity; anagen; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; regulation of glycogen biosynthetic process; response to hormone stimulus; negative regulation of cell size; glucose transport; negative regulation of caspase activity; signal transduction; regulation of translation; apoptotic mitochondrial changes; transport; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; inflammatory response; activated T cell apoptosis; aging; negative regulation of proteolysis; myelin maintenance in the peripheral nervous system; cytoskeleton organization and biogenesis; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; insulin receptor signaling pathway; positive regulation of vasoconstriction; positive regulation of protein amino acid phosphorylation; phosphorylation