ID2 sirna

ID2 siRNA (Mouse)

Cat. Num. AAA8220793

• Gene Names: Id2; Idb2; C78922; bHLHb26; AI255428
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ID2; ID2 siRNA (Mouse); ID-2; IDB2; DNA-binding protein inhibitor ID-2; Inhibitor of DNA binding 2; ID2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ID2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 134

• Applicable Applications for ID2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ID2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ID2 sirna

siRNA to inhibit ID2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6754276
• NCBI GeneID: 15902
• NCBI Accession #: NP_034626.1
• NCBI GenBank Nucleotide #: NM_010496.3
• UniProt Accession #: P41136; O88604
• Molecular Weight: 14,959 Da
• NCBI Official Full Name: DNA-binding protein inhibitor ID-2
• NCBI Official Synonym Full Names: inhibitor of DNA binding 2
• NCBI Official Symbol: Id2
• NCBI Official Synonym Symbols: Idb2; C78922; bHLHb26; AI255428
• NCBI Protein Information: DNA-binding protein inhibitor ID-2
• UniProt Protein Name: DNA-binding protein inhibitor ID-2
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: Id2
• UniProt Synonym Gene Names: Id-2; Idb2
• UniProt Entry Name: ID2_MOUSE

Uniprot Description

ID2: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5. Interacts with NR0B2. Highly expressed in early fetal tissues, including those of the central nervous system.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; protein complex; cytoplasm; nucleus; chromatin; cytosol

Molecular Function: protein dimerization activity; protein binding

Biological Process: regulation of lipid metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; heart development; multicellular organismal development; cell maturation; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; entrainment of circadian clock by photoperiod; locomotor rhythm; regulation of transcription, DNA-dependent; embryonic digestive tract morphogenesis; natural killer cell differentiation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; circadian regulation of gene expression; oligodendrocyte development; positive regulation of macrophage differentiation; positive regulation of astrocyte differentiation; Peyer's patch development; negative regulation of B cell differentiation; transcription, DNA-dependent; olfactory bulb development; positive regulation of erythrocyte differentiation; negative regulation of transcription factor activity; leukocyte differentiation; neuron fate commitment; mammary gland epithelial cell proliferation; positive regulation of cell cycle; enucleate erythrocyte differentiation; regulation of circadian rhythm; lymph node development; negative regulation of DNA binding; negative regulation of oligodendrocyte differentiation; negative regulation of neuron differentiation; positive regulation of fat cell differentiation; entrainment of circadian clock; negative regulation of transcription, DNA-dependent; metanephros development; cell development; positive regulation of blood pressure