MAGEB2 sirna

MAGEB2 siRNA (Human)

Cat. Num. AAA8220781

• Gene Names: MAGEB2; DAM6; CT3.2; MAGE-XP-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAGEB2; MAGEB2 siRNA (Human); Melanoma-associated antigen B2; Cancer/testis antigen 3.2; CT3.2; DSS-AHC critical interval MAGE superfamily 6; DAM6; MAGE XP-2 antigen; MAGE-B2 antigen; MAGEB2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAGEB2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 319

• Applicable Applications for MAGEB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAGEB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAGEB2 sirna

siRNA to inhibit MAGEB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 222418639
• NCBI GeneID: 4113
• NCBI Accession #: NP_002355.2
• NCBI GenBank Nucleotide #: NM_002364.4
• UniProt Accession #: O15479; O75860; AAB50665
• Molecular Weight: 35,277 Da
• NCBI Official Full Name: melanoma-associated antigen B2
• NCBI Official Synonym Full Names: melanoma antigen family B2
• NCBI Official Symbol: MAGEB2
• NCBI Official Synonym Symbols: DAM6; CT3.2; MAGE-XP-2
• NCBI Protein Information: melanoma-associated antigen B2
• UniProt Protein Name: Melanoma-associated antigen B2
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEB2
• UniProt Synonym Gene Names: CT3.2; DAM6
• UniProt Entry Name: MAGB2_HUMAN

NCBI Description

This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region. It is expressed in testis and placenta, and in a significant fraction of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGE-B2: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xp21.3