PITPNM1 sirna

PITPNM1 siRNA (Mouse)

Cat. Num. AAA8220645

• Gene Names: Pitpnm1; Rd9; RdgB; DRES9; Mpt-1; Nir-2; RdgB1; Pitpnm; R75447; PITPnm 1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PITPNM1; PITPNM1 siRNA (Mouse); DRES9; MPT1; NIR2; PITPNM; Membrane-associated phosphatidylinositol transfer protein 1; Drosophila retinal degeneration B homolog 1; RdgB1; Mpt-1; Phosphatidylinositol transfer protein. membrane-associated 1; PITPnm 1; Pyk2 N-terminal domain-interacting receptor 2; NIR-2; PITPNM1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PITPNM1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1243

• Applicable Applications for PITPNM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PITPNM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PITPNM1 sirna

siRNA to inhibit PITPNM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6679339
• NCBI GeneID: 18739
• NCBI Accession #: NP_032877.1
• NCBI GenBank Nucleotide #: NM_008851.4
• UniProt Accession #: O35954
• Molecular Weight: 134,940 Da
• NCBI Official Full Name: membrane-associated phosphatidylinositol transfer protein 1
• NCBI Official Synonym Full Names: phosphatidylinositol transfer protein, membrane-associated 1
• NCBI Official Symbol: Pitpnm1
• NCBI Official Synonym Symbols: Rd9; RdgB; DRES9; Mpt-1; Nir-2; RdgB1; Pitpnm; R75447; PITPnm 1
• NCBI Protein Information: membrane-associated phosphatidylinositol transfer protein 1
• UniProt Protein Name: Membrane-associated phosphatidylinositol transfer protein 1
• Protein Family: Membrane-associated phosphatidylinositol transfer protein
• UniProt Gene Name: Pitpnm1
• UniProt Synonym Gene Names: Dres9; Mpt1; Nir2; Pitpnm; RdgB1; PITPnm 1; NIR-2
• UniProt Entry Name: PITM1_MOUSE

Uniprot Description

PITPNM1: putatively transfers phosphatidylinositol and phosphatidylcholine between membranes. Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Has higher affinity for the inactive, GDP-bound form of RHOA. Necessary for normal completion of cytokinesis and for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions. Interacts with PIK4CA and with Pyk2 via its C-terminus. Phosphorylated on multiple sites by CDC2 at the onset of mitosis. CDC2-phosphorylated forms may interact with PLK1. Interacts with VAPB. Phosphorylated on threonine residues upon treatment with oleic acid.

Protein type: Cell surface

Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; cytoplasm; lipid particle; intracellular

Molecular Function: metal ion binding; phospholipid binding

Biological Process: protein transport; transport