KMT2D sirna

KMT2D siRNA (Human)

Cat. Num. AAA8220441

• Gene Names: KMT2D; ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KMT2D; KMT2D siRNA (Human); ALR; MLL2; MLL4; Histone-lysine N-methyltransferase 2D; Lysine N-methyltransferase 2D; ALL1-related protein; Myeloid/lymphoid or mixed-lineage leukemia protein 2; KMT2D sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KMT2D siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 5537

• Applicable Applications for KMT2D sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KMT2D gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KMT2D sirna

siRNA to inhibit KMT2D expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148762969
• NCBI GeneID: 8085
• NCBI Accession #: NP_003473.3
• NCBI GenBank Nucleotide #: NM_003482.3
• UniProt Accession #: O14686; O14687
• Molecular Weight: 593,677 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase 2D
• NCBI Official Synonym Full Names: lysine (K)-specific methyltransferase 2D
• NCBI Official Symbol: KMT2D
• NCBI Official Synonym Symbols: ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
• NCBI Protein Information: histone-lysine N-methyltransferase 2D
• UniProt Protein Name: Histone-lysine N-methyltransferase 2D
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: KMT2D
• UniProt Synonym Gene Names: ALR; MLL2; MLL4; Lysine N-methyltransferase 2D
• UniProt Entry Name: KMT2D_HUMAN

NCBI Description

The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010]

Uniprot Description

MLL4: Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Defects in MLL2 are the cause of Kabuki syndrome type 1 (KABUK1). A congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 2.1.1.43; Transcription regulation; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific)

Biological Process: oogenesis; oocyte growth; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to estrogen stimulus; histone H3-K4 methylation; positive regulation of estrogen receptor signaling pathway; chromatin silencing; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter

Disease: Kabuki Syndrome 1