AHCYL1 sirna

AHCYL1 siRNA (Human)

Cat. Num. AAA8220364

• Gene Names: AHCYL1; DCAL; IRBIT; PPP1R78; PRO0233; XPVKONA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AHCYL1; AHCYL1 siRNA (Human); DCAL; XPVKONA; Putative adenosylhomocysteinase 2; AdoHcyase 2; DC-expressed AHCY-like molecule; S-adenosyl-L-homocysteine hydrolase 2; S-adenosylhomocysteine hydrolase-like protein 1; AHCYL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AHCYL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 483

• Applicable Applications for AHCYL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AHCYL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AHCYL1 sirna

siRNA to inhibit AHCYL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 336455095
• NCBI GeneID: 10768
• NCBI Accession #: NP_001229602.1
• NCBI GenBank Nucleotide #: NM_001242673.1
• UniProt Accession #: O43865; Q2TAJ6; Q502W8; Q5VSM0; Q6P171; Q96PK4; Q9UG84; B4E168
• Molecular Weight: 53,753 Da
• NCBI Official Full Name: adenosylhomocysteinase 2 isoform b
• NCBI Official Synonym Full Names: adenosylhomocysteinase-like 1
• NCBI Official Symbol: AHCYL1
• NCBI Official Synonym Symbols: DCAL; IRBIT; PPP1R78; PRO0233; XPVKONA
• NCBI Protein Information: adenosylhomocysteinase 2
• UniProt Protein Name: Adenosylhomocysteinase 2
• Protein Family: Adenosylhomocysteinase
• UniProt Gene Name: AHCYL1
• UniProt Synonym Gene Names: AdoHcyase 2; IRBIT
• UniProt Entry Name: SAHH2_HUMAN

NCBI Description

The protein encoded by this gene interacts with inositol 1,4,5-trisphosphate receptor, type 1 and may be involved in the conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

AHCYL1: an inositol 1,4,5-trisphosphate receptor 1 (IP3R1)-binding protein that inhibits IP3 binding to IP3R1 and IP3-induced intracellular Ca2 release. Suppresses the activation of IP3R1 via a phosphorylation-dependent mechanism. Also binds the Na/HCO3 cotransporter 1 (SLC4A4) and may play an important role in pH regulation. Contains a domain homologous to S-adenosylhomocysteine hydrolase (SAHH), although it is reported to possess no hydrolase activity. Its expression is induced in cultured monocytes when they are differentiated into dendritic cells (DC) by exposure to GM-CSF and IL-4.

Protein type: Hydrolase; Amino Acid Metabolism - cysteine and methionine; Other Amino Acids Metabolism - selenoamino acid; EC 3.3.1.1

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: endoplasmic reticulum; cytosol

Molecular Function: protein binding; adenosylhomocysteinase activity

Biological Process: S-adenosylmethionine cycle; regulation of ion transmembrane transporter activity; mRNA polyadenylation; one-carbon compound metabolic process; protein export from nucleus; regulation of mRNA 3'-end processing