TSTA3 sirna

TSTA3 siRNA (Human)

Cat. Num. AAA8220151

• Gene Names: TSTA3; FX; P35B; SDR4E1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TSTA3; TSTA3 siRNA (Human); SDR4E1; GDP-L-fucose synthase; GDP-4-keto-6-deoxy-D-mannose-3.5-epimerase-4-reductase; Protein FX; Red cell NADP(H)-binding protein; Short-chain dehydrogenase/reductase family 4E member 1; TSTA3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TSTA3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 321

• Applicable Applications for TSTA3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TSTA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TSTA3 sirna

siRNA to inhibit TSTA3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4507709
• NCBI GeneID: 7264
• NCBI Accession #: NP_003304.1
• NCBI GenBank Nucleotide #: NM_003313.3
• UniProt Accession #: Q13630; Q567Q9; Q9UDG7; B2R8Y7; D3DWK5
• Molecular Weight: 35,893 Da
• NCBI Official Full Name: GDP-L-fucose synthase
• NCBI Official Synonym Full Names: tissue specific transplantation antigen P35B
• NCBI Official Symbol: TSTA3
• NCBI Official Synonym Symbols: FX; P35B; SDR4E1
• NCBI Protein Information: GDP-L-fucose synthase
• UniProt Protein Name: GDP-L-fucose synthase
• UniProt Gene Name: TSTA3
• UniProt Synonym Gene Names: SDR4E1
• UniProt Entry Name: FCL_HUMAN

NCBI Description

Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. [provided by RefSeq, Jul 2008]

Uniprot Description

TSTA3: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. Belongs to the fucose synthase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 1.1.1.271; Oxidoreductase

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm

Molecular Function: GDP-L-fucose synthase activity; electron carrier activity; isomerase activity; coenzyme binding; GDP-4-dehydro-D-rhamnose reductase activity

Biological Process: leukocyte adhesion; cytolysis; GDP-mannose metabolic process; 'de novo' GDP-L-fucose biosynthetic process