PTPDC1 sirna

PTPDC1 siRNA (Mouse)

Cat. Num. AAA8220007

• Gene Names: Ptpdc1; Naa-1; Ptpcd1; AI843923; AW456874
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTPDC1; PTPDC1 siRNA (Mouse); Protein tyrosine phosphatase domain-containing protein 1; PTPDC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PTPDC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 808

• Applicable Applications for PTPDC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTPDC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTPDC1 sirna

siRNA to inhibit PTPDC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 684625054
• NCBI GeneID: 218232
• NCBI Accession #: NP_001288710.1
• NCBI GenBank Nucleotide #: NM_001301781.1
• UniProt Accession #: Q6NZK8; Q3V0Z6; Q3V3K9; Q3V3Y0; Q6NV81; Q8BKD9
• Molecular Weight: 90,964 Da
• NCBI Official Full Name: protein tyrosine phosphatase domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase domain containing 1
• NCBI Official Symbol: Ptpdc1
• NCBI Official Synonym Symbols: Naa-1; Ptpcd1; AI843923; AW456874
• NCBI Protein Information: protein tyrosine phosphatase domain-containing protein 1
• UniProt Protein Name: Protein tyrosine phosphatase domain-containing protein 1
• Protein Family: Protein tyrosine phosphatase domain-containing protein
• UniProt Gene Name: Ptpdc1
• UniProt Entry Name: PTPC1_MOUSE

NCBI Description

The protein encoded by this gene is a centrosomal mitotic phosphatase. This protein has been implicated in centrosomal duplication and cytokinesis. In addition, knockdown of expression levels in non-cycling cells results in extra long cilia, suggesting that this protein may function in regulating cilia length independent of a function in cell cycle control. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]

Uniprot Description

PTPDC1: May play roles in cilia formation and/or maintenance. Belongs to the protein-tyrosine phosphatase family. Non-receptor class PTPDC1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.-; Protein phosphatase, tyrosine (non-receptor)

Cellular Component: nucleoplasm

Molecular Function: protein tyrosine/threonine phosphatase activity; inositol-1,3,4-trisphosphate 4-phosphatase activity; phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity; hydrolase activity; protein tyrosine phosphatase activity; inositol-1,4,5,6-tetrakisphosphate 6-phosphatase activity; inositol bisphosphate phosphatase activity; inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity; transmembrane receptor protein phosphatase activity; JUN kinase phosphatase activity; NADP phosphatase activity; 5-amino-6-(5-phosphoribitylamino)uracil phosphatase activity; phosphatidylinositol-4-phosphate phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; phosphoric monoester hydrolase activity; inositol-4,5-bisphosphate 5-phosphatase activity; phosphohistidine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: smoothened signaling pathway; cell projection organization and biogenesis; dephosphorylation; protein amino acid dephosphorylation