ENO1 sirna

ENO1 siRNA (Human)

Cat. Num. AAA8219962

• Gene Names: ENO1; NNE; PPH; MPB1; ENO1L1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ENO1; ENO1 siRNA (Human); ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein; ENO1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ENO1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 341

• Applicable Applications for ENO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ENO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Ships on blue ice.; Store at -20°C for up to one year.

Related Product Information for ENO1 sirna

siRNA to inhibit ENO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 319996655
• NCBI GeneID: 2023
• NCBI Accession #: NP_001188412.1
• NCBI GenBank Nucleotide #: NM_001201483.1
• UniProt Accession #: P06733; P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
• NCBI Official Full Name: c-myc promoter-binding protein-1 isoform MBP-1
• NCBI Official Synonym Full Names: enolase 1, (alpha)
• NCBI Official Symbol: ENO1
• NCBI Official Synonym Symbols: NNE; PPH; MPB1; ENO1L1
• NCBI Protein Information: alpha-enolase; c-myc promoter-binding protein-1
• UniProt Protein Name: Alpha-enolase
• Protein Family: Enolase
• UniProt Gene Name: ENO1
• UniProt Synonym Gene Names: ENO1L1; MBPB1; MPB1; NNE
• UniProt Entry Name: ENOA_HUMAN

NCBI Description

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Uniprot Description

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; phosphopyruvate hydratase complex; membrane; cytoplasm; plasma membrane; M band; cytosol; nucleus

Molecular Function: protein binding; DNA binding; magnesium ion binding; phosphopyruvate hydratase activity; transcription factor activity; transcription corepressor activity

Biological Process: glycolysis; transcription, DNA-dependent; response to virus; carbohydrate metabolic process; glucose metabolic process; pathogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; gluconeogenesis