RNF5 sirna

RNF5 siRNA (Human)

Cat. Num. AAA8219408

• Gene Names: RNF5; RMA1; RING5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF5; RNF5 siRNA (Human); G16; NG2; RMA1; E3 ubiquitin-protein ligase RNF5; Protein G16; RING finger protein 5; Ram1 homolog; HsRma1; RNF5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RNF5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 180

• Applicable Applications for RNF5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RNF5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF5 sirna

siRNA to inhibit RNF5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5902054
• NCBI GeneID: 6048
• NCBI Accession #: NP_008844.1
• NCBI GenBank Nucleotide #: NM_006913.3
• UniProt Accession #: Q99942; Q0VDB7; Q9UMQ2; A2BFI6; B2R4K3
• Molecular Weight: 19,881 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF5
• NCBI Official Synonym Full Names: ring finger protein 5, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF5
• NCBI Official Synonym Symbols: RMA1; RING5
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF5
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF5
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF5
• UniProt Synonym Gene Names: G16; NG2; RMA1; HsRma1
• UniProt Entry Name: RNF5_HUMAN

NCBI Description

The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF5: Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Membrane protein, integral; Membrane protein, multi-pass; EC 6.3.2.-; EC 6.3.2.19; Ligase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: endoplasmic reticulum membrane; mitochondrial membrane; integral to membrane

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: negative regulation of autophagy; protein destabilization; response to bacterium; cellular protein catabolic process